Seroatlas · Human Serome Atlas

ANO9

Anoctamin-9

Also known as: ANO9_HUMAN, PIG5, TMEM16J, TP53I5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
A1A5B4
Gene
ANO9
Ensembl
ENSG00000185101
Chromosome
11
Canonical length
782 aa
Protein class
Predicted membrane proteins, Transporters
Subcellular location
Golgi apparatus

OverviewNCBI Gene

The protein encoded by this gene is a member of the TMEM16 (anoctamin) family of proteins, some of which form integral membrane calcium-activated chloride channels. The function of the encoded protein has yet to be elucidated, although it may have channel-forming abilities and also may have phospholipid scramblase activity. This gene has been observed to be upregulated in stage II and III colorectal cancers. [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

782 residues, UniProt reviewed canonical sequence.

>A1A5B4|ANO9
     1  MQGEESLRIL VEPEGDSFPL MEISTCETEA SEQWDYVLVA QRHTQRDPRQ ARQQQFLEEL
    61  RRKGFHIKVI RDQKQVFFGI RADNSVFGLY RTLLLEPEGP APHAELAAPT TIPVTTSLRI
   121  RIVNFVVMNN KTSAGETFED LMKDGVFEAR FPLHKGEGRL KKTWARWRHM FREQPVDEIR
   181  NYFGEKVALY FVWLGWYTYM LVPAALTGLL VFLSGFSLFE ASQISKEICE AHDILMCPLG
   241  DHSRRYQRLS ETCTFAKLTH LFDNDGTVVF AIFMALWATV FLEIWKRQRA RVVLHWDLYV
   301  WDEEQEEMAL QLINCPDYKL RPYQHSYLRS TVILVLTLLM ICLMIGMAHV LVVYRVLASA
   361  LFSSSAVPFL EEQVTTAVVV TGALVHYVTI IIMTKINRCV ALKLCDFEMP RTFSERESRF
   421  TIRFFTLQFF THFSSLIYIA FILGRINGHP GKSTRLAGLW KLEECHASGC MMDLFVQMAI
   481  IMGLKQTLSN CVEYLVPWVT HKCRSLRASE SGHLPRDPEL RDWRRNYLLN PVNTFSLFDE
   541  FMEMMIQYGF TTIFVAAFPL APLLALFSNL VEIRLDAIKM VWLQRRLVPR KAKDIGTWLQ
   601  VLETIGVLAV IANGMVIAFT SEFIPRVVYK YRYSPCLKEG NSTVDCLKGY VNHSLSVFHT
   661  KDFQDPDGIE GSENVTLCRY RDYRNPPDYN FSEQFWFLLA IRLAFVILFE HVALCIKLIA
   721  AWFVPDIPQS VKNKVLEVKY QRLREKMWHG RQRLGGVGAG SRPPMPAHPT PASIFSARST
   781  DV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANO9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
8
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
32 nTPM

Expression across tissuesHPA

Tissue

  • duodenum: 32 nTPM
  • small intestine: 29 nTPM
  • skin: 26 nTPM
  • colon: 20 nTPM
  • lymph node: 11 nTPM
  • rectum: 8.6 nTPM

Single-cell type

  • enterocytes: 110 nCPM
  • colonocytes: 82 nCPM
  • goblet cells: 77 nCPM
  • paneth cells: 66 nCPM
  • enteric transient amplifying cells: 53 nCPM
  • enteric stem cells: 49 nCPM

Immune cell

  • T-reg: 8.3 nTPM
  • memory CD4 T-cell: 6.5 nTPM
  • MAIT T-cell: 5.5 nTPM
  • NK-cell: 4.1 nTPM
  • naive CD4 T-cell: 3.7 nTPM
  • memory CD8 T-cell: 3.3 nTPM

Brain region

  • choroid plexus: 0.4 nTPM
  • basal ganglia: 0.1 nTPM
  • cerebral cortex: 0.1 nTPM
  • medulla oblongata: 0.1 nTPM
  • pons: 0.1 nTPM
  • spinal cord: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.91
gnomAD pLI
0
gnomAD missense Z
1.21
DepMap mean gene effect
0.15
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANO9 as an antibody target. Whether an autoantibody or antibody against ANO9 could matter depends on whether native ANO9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANO9 is annotated at the cell surface, where native ANO9 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ANO9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANO9. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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