Seroatlas · Human Serome Atlas

ANO4

Anoctamin-4

Also known as: ANO4_HUMAN, FLJ34221, FLJ34272, FLJ35277, TMEM16D

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q32M45
Gene: ANO4
Ensembl: ENSG00000151572
Chromosome: 12
Canonical length: 955 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

Enables intracellularly calcium-gated chloride channel activity. Involved in chloride transmembrane transport. Located in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

955 residues, UniProt reviewed canonical sequence.

>Q32M45|ANO4
     1  MEASSSGITN GKTKVFHPEG GVDLQGYQLD MQILPDGPKS DVDFSEILNA IQEMAKDVNI
    61  LFDELEAVSS PCKDDDSLLH PGNLTSTSDD ASRLEAGGET VPERNKSNGL YFRDGKCRID
   121  YILVYRKSNP QTEKREVFER NIRAEGLQME KESSLINSDI IFVKLHAPWE VLGRYAEQMN
   181  VRMPFRRKIY YLPRRYKFMS RIDKQISRFR RWLPKKPMRL DKETLPDLEE NDCYTAPFSQ
   241  QRIHHFIIHN KETFFNNATR SRIVHHILQR IKYEEGKNKI GLNRLLTNGS YEAAFPLHEG
   301  SYRSKNSIRT HGAENHRHLL YECWASWGVW YKYQPLDLVR RYFGEKIGLY FAWLGWYTGM
   361  LFPAAFIGLF VFLYGVTTLD HSQVSKEVCQ ATDIIMCPVC DKYCPFMRLS DSCVYAKVTH
   421  LFDNGATVFF AVFMAVWATV FLEFWKRRRA VIAYDWDLID WEEEEEEIRP QFEAKYSKKE
   481  RMNPISGKPE PYQAFTDKCS RLIVSASGIF FMICVVIAAV FGIVIYRVVT VSTFAAFKWA
   541  LIRNNSQVAT TGTAVCINFC IIMLLNVLYE KVALLLTNLE QPRTESEWEN SFTLKMFLFQ
   601  FVNLNSSTFY IAFFLGRFTG HPGAYLRLIN RWRLEECHPS GCLIDLCMQM GIIMVLKQTW
   661  NNFMELGYPL IQNWWTRRKV RQEHGPERKI SFPQWEKDYN LQPMNAYGLF DEYLEMILQF
   721  GFTTIFVAAF PLAPLLALLN NIIEIRLDAY KFVTQWRRPL ASRAKDIGIW YGILEGIGIL
   781  SVITNAFVIA ITSDFIPRLV YAYKYGPCAG QGEAGQKCMV GYVNASLSVF RISDFENRSE
   841  PESDGSEFSG TPLKYCRYRD YRDPPHSLVP YGYTLQFWHV LAARLAFIIV FEHLVFCIKH
   901  LISYLIPDLP KDLRDRMRRE KYLIQEMMYE AELERLQKER KERKKNGKAH HNEWP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANO4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 8
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

adrenal gland: 11 nTPM
seminal vesicle: 7.6 nTPM
cervix: 7.2 nTPM
cerebral cortex: 5 nTPM
prostate: 4.5 nTPM
endometrium: 4.1 nTPM

Single-cell type

oligodendrocytes: 941 nCPM
adrenal medulla cells: 489 nCPM
oligodendrocyte progenitor cells: 453 nCPM
brain inhibitory neurons: 289 nCPM
retinal ganglion cells: 251 nCPM
epididymal clear cells: 227 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 34 nTPM
basal ganglia: 32 nTPM
thalamus: 30 nTPM
cerebral cortex: 29 nTPM
midbrain: 25 nTPM
medulla oblongata: 24 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ANO4.

Disease | GeneticClinVar

7 pathogenic / likely-pathogenic of 112 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Developmental and epileptic encephalopathy
Generalized epilepsy with febrile seizures plus
Temporal lobe epilepsy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.44
gnomAD pLI: 0
gnomAD missense Z: 2.53
DepMap mean gene effect: -0.04
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

plasma membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANO4 as an antibody target. Whether an autoantibody or antibody against ANO4 could matter depends on whether native ANO4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANO4 is annotated at the cell surface, where native ANO4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ANO4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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