ANKLE1

Protein identityUniProt · HPA

UniProt accession

Q8NAG6

Gene

ANKLE1

Ensembl

ENSG00000160117

Chromosome

19

Canonical length

615 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables endonuclease activity. Involved in DNA damage response and protein export from nucleus. Located in cytosol and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

615 residues, UniProt reviewed canonical sequence.

>Q8NAG6|ANKLE1
     1  MCSEARLARR LRDALREEEP WAVEELLRCG ADPNLVLEDG AAAVHLAAGA RHPRGLRCLG
    61  ALLRQGGDPN ARSVEALTPL HVAAAWGCRR GLELLLSQGA DPALRDQDGL RPLDLALQQG
   121  HLECARVLQD LDTRTRTRTR IGAETQEPEP APGTPGLSGP TDETLDSIAL QKQPCRGDNR
   181  DIGLEADPGP PSLPVPLETV DKHGSSASPP GHWDYSSDAS FVTAVEVSGA EDPASDTPPW
   241  AGSLPPTRQG LLHVVHANQR VPRSQGTEAE LNARLQALTL TPPNAAGFQS SPSSMPLLDR
   301  SPAHSPPRTP TPGASDCHCL WEHQTSIDSD MATLWLTEDE ASSTGGREPV GPCRHLPVST
   361  VSDLELLKGL RALGENPHPI TPFTRQLYHQ QLEEAQIAPG PEFSGHSLEL AAALRTGCIP
   421  DVQADEDALA QQFEQPDPAR RWREGVVKSS FTYLLLDPRE TQDLPARAFS LTPAERLQTF
   481  IRAIFYVGKG TRARPYVHLW EALGHHGRSR KQPHQACPKV RQILDIWASG CGVVSLHCFQ
   541  HVVAVEAYTR EACIVEALGI QTLTNQKQGH CYGVVAGWPP ARRRRLGVHL LHRALLVFLA
   601  EGERQLHPQD IQARG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKLE1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.43

Highest tissue expression

23 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.44

gnomAD pLI

0

gnomAD missense Z

0.86

DepMap mean gene effect

-0.13

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA damage response
• double-strand break repair via homologous recombination
• negative regulation of mitotic recombination
• nucleic acid metabolic process
• protein export from nucleus
• regulation of myeloid progenitor cell differentiation
• resolution of meiotic recombination intermediates
• regulation of lymphoid progenitor cell differentiation

Molecular functions

• cobalt ion binding
• DNA endonuclease activity
• endonuclease activity
• hydrolase activity
• magnesium ion binding
• manganese ion binding

Cellular components

• cytoplasm
• cytosol
• midbody
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• GIY-YIG endonuclease
• Ankyrin repeat
• LEM domain
• LEM/LEM-like domain superfamily
• Ankyrin repeat-containing domain superfamily
• Ankyrin repeats (3 copies)
• Ankyrin repeat and LEM domain-containing protein 1
• LEM-3-like GIY-YIG domain

KeywordsUniProt

• ANK repeat
• Cell cycle
• Cell division
• Cytoplasm
• DNA damage
• DNA repair
• Endonuclease
• Hydrolase
• Nuclease
• Nucleus
• Repeat

InteractionsUniProt · HPA

Protein binding partners of ANKLE1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BANF1