Seroatlas · Human Serome Atlas

ALOX5AP

Arachidonate 5-lipoxygenase-activating protein

Also known as: AL5AP_HUMAN, FLAP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P20292
Gene
ALOX5AP
Ensembl
ENSG00000132965
Chromosome
13
Canonical length
161 aa
Protein class
Disease related genes, Predicted membrane proteins
Quaternary structure
Homotrimer

OverviewNCBI Gene

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

161 residues, UniProt reviewed canonical sequence.

>P20292|ALOX5AP
     1  MDQETVGNVV LLAIVTLISV VQNGFFAHKV EHESRTQNGR SFQRTGTLAF ERVYTANQNC
    61  VDAYPTFLAV LWSAGLLCSQ VPAAFAGLMY LFVRQKYFVG YLGERTQSTP GYIFGKRIIL
   121  FLFLMSVAGI FNYYLIFFFG SDFENYIKTI STTISPLLLI P

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALOX5AP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
460 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 460 nTPM
  • lung: 173 nTPM
  • spleen: 142 nTPM
  • tonsil: 101 nTPM
  • appendix: 95 nTPM
  • thymus: 87 nTPM

Single-cell type

  • neutrophils: 3,929 nCPM
  • neutrophil progenitors: 1,950 nCPM
  • mast cells: 943 nCPM
  • pdcs: 775 nCPM
  • hofbauer cells: 560 nCPM
  • tuft cells: 392 nCPM

Immune cell

  • basophil: 9,739 nTPM
  • neutrophil: 4,992 nTPM
  • eosinophil: 4,044 nTPM
  • plasmacytoid DC: 3,564 nTPM
  • T-reg: 847 nTPM
  • MAIT T-cell: 810 nTPM

Brain region

  • white matter: 54 nTPM
  • medulla oblongata: 32 nTPM
  • cerebral cortex: 27 nTPM
  • thalamus: 26 nTPM
  • pons: 26 nTPM
  • spinal cord: 23 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ALOX5AP.

Disease | AllUniProt

Conditions ALOX5AP is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.96
gnomAD pLI
0.07
gnomAD missense Z
1.04
DepMap mean gene effect
0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ALOX5AP in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ALOX5AP as an antibody target. Whether an autoantibody or antibody against ALOX5AP could matter depends on whether native ALOX5AP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ALOX5AP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ALOX5AP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ALOX5AP. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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