AHNAK

Protein identityUniProt · HPA

UniProt accession

Q09666

Gene

AHNAK

Ensembl

ENSG00000124942

Chromosome

11

Canonical length

5890 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene. [provided by RefSeq, Oct 2016]

Canonical amino-acid sequenceUniProt

5890 residues, UniProt reviewed canonical sequence.

>Q09666|AHNAK
     1  MEKEETTREL LLPNWQGSGS HGLTIAQRDD GVFVQEVTQN SPAARTGVVK EGDQIVGATI
    61  YFDNLQSGEV TQLLNTMGHH TVGLKLHRKG DRSPEPGQTW TREVFSSCSS EVVLSGDDEE
   121  YQRIYTTKIK PRLKSEDGVE GDLGETQSRT ITVTRRVTAY TVDVTGREGA KDIDISSPEF
   181  KIKIPRHELT EISNVDVETQ SGKTVIRLPS GSGAASPTGS AVDIRAGAIS ASGPELQGAG
   241  HSKLQVTMPG IKVGGSGVNV NAKGLDLGGR GGVQVPAVDI SSSLGGRAVE VQGPSLESGD
   301  HGKIKFPTMK VPKFGVSTGR EGQTPKAGLR VSAPEVSVGH KGGKPGLTIQ APQLEVSVPS
   361  ANIEGLEGKL KGPQITGPSL EGDLGLKGAK PQGHIGVDAS APQIGGSITG PSVEVQAPDI
   421  DVQGPGSKLN VPKMKVPKFS VSGAKGEETG IDVTLPTGEV TVPGVSGDVS LPEIATGGLE
   481  GKMKGTKVKT PEMIIQKPKI SMQDVDLSLG SPKLKGDIKV SAPGVQGDVK GPQVALKGSR
   541  VDIETPNLEG TLTGPRLGSP SGKTGTCRIS MSEVDLNVAA PKVKGGVDVT LPRVEGKVKV
   601  PEVDVRGPKV DVSAPDVEAH GPEWNLKMPK MKMPTFSTPG AKGEGPDVHM TLPKGDISIS
   661  GPKVNVEAPD VNLEGLGGKL KGPDVKLPDM SVKTPKISMP DVDLHVKGTK VKGEYDVTVP
   721  KLEGELKGPK VDIDAPDVDV HGPDWHLKMP KMKMPKFSVP GFKAEGPEVD VNLPKADVDI
   781  SGPKIDVTAP DVSIEEPEGK LKGPKFKMPE MNIKVPKISM PDVDLHLKGP NVKGEYDVTM
   841  PKVESEIKVP DVELKSAKMD IDVPDVEVQG PDWHLKMPKM KMPKFSMPGF KAEGPEVDVN
   901  LPKADVDISG PKVGVEVPDV NIEGPEGKLK GPKFKMPEMN IKAPKISMPD VDLHMKGPKV
   961  KGEYDMTVPK LEGDLKGPKV DVSAPDVEMQ GPDWNLKMPK IKMPKFSMPS LKGEGPEFDV
  1021  NLSKANVDIS APKVDTNAPD LSLEGPEGKL KGPKFKMPEM HFRAPKMSLP DVDLDLKGPK
  1081  MKGNVDISAP KIEGEMQVPD VDIRGPKVDI KAPDVEGQGL DWSLKIPKMK MPKFSMPSLK
  1141  GEGPEVDVNL PKADVVVSGP KVDIEAPDVS LEGPEGKLKG PKFKMPEMHF KTPKISMPDV
  1201  DLHLKGPKVK GDVDVSVPKV EGEMKVPDVE IKGPKMDIDA PDVEVQGPDW HLKMPKMKMP
  1261  KFSMPGFKGE GREVDVNLPK ADIDVSGPKV DVEVPDVSLE GPEGKLKGPK FKMPEMHFKA
  1321  PKISMPDVDL NLKGPKLKGD VDVSLPEVEG EMKVPDVDIK GPKVDISAPD VDVHGPDWHL
  1381  KMPKVKMPKF SMPGFKGEGP EVDVKLPKAD VDVSGPKMDA EVPDVNIEGP DAKLKGPKFK
  1441  MPEMSIKPQK ISIPDVGLHL KGPKMKGDYD VTVPKVEGEI KAPDVDIKGP KVDINAPDVE
  1501  VHGPDWHLKM PKVKMPKFSM PGFKGEGPEV DMNLPKADLG VSGPKVDIDV PDVNLEAPEG
  1561  KLKGPKFKMP SMNIQTHKIS MPDVGLNLKA PKLKTDVDVS LPKVEGDLKG PEIDVKAPKM
  1621  DVNVGDIDIE GPEGKLKGPK FKMPEMHFKA PKISMPDVDL HLKGPKVKGD MDVSVPKVEG
  1681  EMKVPDVDIK GPKVDIDAPD VEVHDPDWHL KMPKMKMPKF SMPGFKAEGP EVDVNLPKAD
  1741  IDVSGPSVDT DAPDLDIEGP EGKLKGSKFK MPKLNIKAPK VSMPDVDLNL KGPKLKGEID
  1801  ASVPELEGDL RGPQVDVKGP FVEAEVPDVD LECPDAKLKG PKFKMPEMHF KAPKISMPDV
  1861  DLHLKGPKVK GDADVSVPKL EGDLTGPSVG VEVPDVELEC PDAKLKGPKF KMPDMHFKAP
  1921  KISMPDVDLH LKGPKVKGDV DVSVPKLEGD LTGPSVGVEV PDVELECPDA KLKGPKFKMP
  1981  EMHFKTPKIS MPDVDLHLKG PKVKGDMDVS VPKVEGEMKV PDVDIKGPKM DIDAPDVDVH
  2041  GPDWHLKMPK MKMPKFSMPG FKAEGPEVDV NLPKADVVVS GPKVDVEVPD VSLEGPEGKL
  2101  KGPKLKMPEM HFKAPKISMP DVDLHLKGPK VKGDVDVSLP KLEGDLTGPS VDVEVPDVEL
  2161  ECPDAKLKGP KFKMPEMHFK TPKISMPDVN LNLKGPKVKG DMDVSVPKVE GEMKVPDVDI
  2221  RGPKVDIDAP DVDVHGPDWH LKMPKMKMPK FSMPGFKGEG PEVDVNLPKA DVDVSGPKVD
  2281  VEVPDVSLEG PEGKLKGPKF KMPEMHFKTP KISMPDVDFN LKGPKIKGDV DVSAPKLEGE
  2341  LKGPELDVKG PKLDADMPEV AVEGPNGKWK TPKFKMPDMH FKAPKISMPD LDLHLKSPKA
  2401  KGEVDVDVPK LEGDLKGPHV DVSGPDIDIE GPEGKLKGPK FKMPDMHFKA PNISMPDVDL
  2461  NLKGPKIKGD VDVSVPEVEG KLEVPDMNIR GPKVDVNAPD VQAPDWHLKM PKMKMPKFSM
  2521  PGFKAEGPEV DVNLPKADVD ISGPKVDIEG PDVNIEGPEG KLKGPKLKMP EMNIKAPKIS
  2581  MPDFDLHLKG PKVKGDVDVS LPKVEGDLKG PEVDIKGPKV DINAPDVGVQ GPDWHLKMPK
  2641  VKMPKFSMPG FKGEGPDGDV KLPKADIDVS GPKVDIEGPD VNIEGPEGKL KGPKFKMPEM
  2701  NIKAPKISMP DIDLNLKGPK VKGDVDVSLP KVEGDLKGPE VDIKGPKVDI DAPDVDVHGP
  2761  DWHLKMPKIK MPKISMPGFK GEGPDVDVNL PKADIDVSGP KVDVECPDVN IEGPEGKWKS
  2821  PKFKMPEMHF KTPKISMPDI DLNLTGPKIK GDVDVTGPKV EGDLKGPEVD LKGPKVDIDV
  2881  PDVNVQGPDW HLKMPKMKMP KFSMPGFKAE GPEVDVNLPK ADVDVSGPKV DVEGPDVNIE
  2941  GPEGKLKGPK FKMPEMNIKA PKIPMPDFDL HLKGPKVKGD VDISLPKVEG DLKGPEVDIR
  3001  GPQVDIDVPD VGVQGPDWHL KMPKVKMPKF SMPGFKGEGP DVDVNLPKAD LDVSGPKVDI
  3061  DVPDVNIEGP EGKLKGPKFK MPEMNIKAPK ISMPDIDLNL KGPKVKGDMD VSLPKVEGDM
  3121  KVPDVDIKGP KVDINAPDVD VQGPDWHLKM PKIKMPKISM PGFKGEGPEV DVNLPKADLD
  3181  VSGPKVDVDV PDVNIEGPDA KLKGPKFKMP EMNIKAPKIS MPDLDLNLKG PKMKGEVDVS
  3241  LANVEGDLKG PALDIKGPKI DVDAPDIDIH GPDAKLKGPK LKMPDMHVNM PKISMPEIDL
  3301  NLKGSKLKGD VDVSGPKLEG DIKAPSLDIK GPEVDVSGPK LNIEGKSKKS RFKLPKFNFS
  3361  GSKVQTPEVD VKGKKPDIDI TGPKVDINAP DVEVQGKVKG SKFKMPFLSI SSPKVSMPDV
  3421  ELNLKSPKVK GDLDIAGPNL EGDFKGPKVD IKAPEVNLNA PDVDVHGPDW NLKMPKMKMP
  3481  KFSVSGLKAE GPDVAVDLPK GDINIEGPSM NIEGPDLNVE GPEGGLKGPK FKMPDMNIKA
  3541  PKISMPDIDL NLKGPKVKGD VDISLPKLEG DLKGPEVDIK GPKVDINAPD VDVHGPDWHL
  3601  KMPKVKMPKF SMPGFKGEGP EVDVTLPKAD IDISGPNVDV DVPDVNIEGP DAKLKGPKFK
  3661  MPEMNIKAPK ISMPDFDLNL KGPKMKGDVV VSLPKVEGDL KGPEVDIKGP KVDIDTPDIN
  3721  IEGSEGKFKG PKFKIPEMHL KAPKISMPDI DLNLKGPKVK GDVDVSLPKM EGDLKGPEVD
  3781  IKGPKVDINA PDVDVQGPDW HLKMPKVKMP KFSMPGFKGE GPDVDVNLPK ADLDVSGPKV
  3841  DIDVPDVNIE GPEGKLKGPK FKMPEMNIKA PKISMPDIDL NLKGPKVKGD MDVSLPKVEG
  3901  DMQVPDLDIK GPKVDINAPD VDVRGPDWHL KMPKIKMPKI SMPGFKGEGP EVDVNLPKAD
  3961  LDVSGPKVDV DVPDVNIEGP DAKLKGPKFK MPEMNIKAPK ISMPDFDLHL KGPKVKGDVD
  4021  VSLPKMEGDL KAPEVDIKGP KVDIDAPDVD VHGPDWHLKM PKVKMPKFSM PGFKGEGPEV
  4081  DVNLPKADID VSGPKVDIDT PDIDIHGPEG KLKGPKFKMP DLHLKAPKIS MPEVDLNLKG
  4141  PKMKGDVDVS LPKVEGDLKG PEVDIKGPKV DIDVPDVDVQ GPDWHLKMPK VKMPKFSMPG
  4201  FKGEGPDVDV NLPKADLDVS GPKVDIDVPD VNIEGPDAKL KGPKFKMPEM NIKAPKISMP
  4261  DFDLHLKGPK VKGDVDVSLP KVEGDLKGPE VDIKGPKVDI DAPDVDVHGP DWHLKMPKVK
  4321  MPKFSMPGFK GEGPDVDVTL PKADIEISGP KVDIDAPDVS IEGPDAKLKG PKFKMPEMNI
  4381  KAPKISMPDI DFNLKGPKVK GDVDVSLPKV EGDLKGPEID IKGPSLDIDT PDVNIEGPEG
  4441  KLKGPKFKMP EMNIKAPKIS MPDFDLHLKG PKVKGDVDVS LPKVESDLKG PEVDIEGPEG
  4501  KLKGPKFKMP DVHFKSPQIS MSDIDLNLKG PKIKGDMDIS VPKLEGDLKG PKVDVKGPKV
  4561  GIDTPDIDIH GPEGKLKGPK FKMPDLHLKA PKISMPEVDL NLKGPKVKGD MDISLPKVEG
  4621  DLKGPEVDIR DPKVDIDVPD VDVQGPDWHL KMPKVKMPKF SMPGFKGEGP DVDVNLPKAD
  4681  IDVSGPKVDV DVPDVNIEGP DAKLKGPKFK MPEMSIKAPK ISMPDIDLNL KGPKVKGDVD
  4741  VTLPKVEGDL KGPEADIKGP KVDINTPDVD VHGPDWHLKM PKVKMPKFSM PGFKGEGPDV
  4801  DVSLPKADID VSGPKVDVDI PDVNIEGPDA KLKGPKFKMP EINIKAPKIS IPDVDLDLKG
  4861  PKVKGDFDVS VPKVEGTLKG PEVDLKGPRL DFEGPDAKLS GPSLKMPSLE ISAPKVTAPD
  4921  VDLHLKAPKI GFSGPKLEGG EVDLKGPKVE APSLDVHMDS PDINIEGPDV KIPKFKKPKF
  4981  GFGAKSPKAD IKSPSLDVTV PEAELNLETP EISVGGKGKK SKFKMPKIHM SGPKIKAKKQ
  5041  GFDLNVPGGE IDASLKAPDV DVNIAGPDAA LKVDVKSPKT KKTMFGKMYF PDVEFDIKSP
  5101  KFKAEAPLPS PKLEGELQAP DLELSLPAIH VEGLDIKAKA PKVKMPDVDI SVPKIEGDLK
  5161  GPKVQANLGA PDINIEGLDA KVKTPSFGIS APQVSIPDVN VNLKGPKIKG DVPSVGLEGP
  5221  DVDLQGPEAK IKFPKFSMPK IGIPGVKMEG GGAEVHAQLP SLEGDLRGPD VKLEGPDVSL
  5281  KGPGVDLPSV NLSMPKVSGP DLDLNLKGPS LKGDLDASVP SMKVHAPGLN LSGVGGKMQV
  5341  GGDGVKVPGI DATTKLNVGA PDVTLRGPSL QGDLAVSGDI KCPKVSVGAP DLSLEASEGS
  5401  IKLPKMKLPQ FGISTPGSDL HVNAKGPQVS GELKGPGVDV NLKGPRISAP NVDFNLEGPK
  5461  VKGSLGATGE IKGPTVGGGL PGIGVQGLEG NLQMPGIKSS GCDVNLPGVN VKLPTGQISG
  5521  PEIKGGLKGS EVGFHGAAPD ISVKGPAFNM ASPESDFGIN LKGPKIKGGA DVSGGVSAPD
  5581  ISLGEGHLSV KGSGGEWKGP QVSSALNLDT SKFAGGLHFS GPKVEGGVKG GQIGLQAPGL
  5641  SVSGPQGHLE SGSGKVTFPK MKIPKFTFSG RELVGREMGV DVHFPKAEAS IQAGAGDGEW
  5701  EESEVKLKKS KIKMPKFNFS KPKGKGGVTG SPEASISGSK GDLKSSKASL GSLEGEAEAE
  5761  ASSPKGKFSL FKSKKPRHRS NSFSDEREFS GPSTPTGTLE FEGGEVSLEG GKVKGKHGKL
  5821  KFGTFGGLGS KSKGHYEVTG SDDETGKLQG SGVSLASKKS RLSSSSSNDS GNKVGIQLPE
  5881  VELSVSTKKE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AHNAK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0

Highest tissue expression

316 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about AHNAK.

Disease | ImmuneIEDB

Conditions an epitope on AHNAK was assayed in.

• skin melanoma T cell

ReferencesPubMed · IEDB

Publications for AHNAK from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.9

gnomAD missense Z

-2.97

DepMap mean gene effect

-0.2

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• positive regulation of plasma membrane repair
• regulation of RNA splicing

Molecular functions

• cadherin binding
• identical protein binding
• RNA binding
• S100 protein binding
• structural molecule activity conferring elasticity

Cellular components

• actin cytoskeleton
• cell-cell contact zone
• costamere
• cytoplasm
• cytosol
• extracellular exosome
• focal adhesion
• lysosomal membrane
• membrane
• nucleus
• plasma membrane
• plasma membrane protein complex
• sarcolemma
• T-tubule
• vesicle

Protein domainsUniProt · Pfam · InterPro

• PDZ domain
• PDZ superfamily
• Peripheral myelin sheath structural protein

KeywordsUniProt

• Acetylation
• Isopeptide bond
• Methylation
• Nucleus
• Phosphoprotein
• Repeat
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of AHNAK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DYSF
• EGFR
• HSPA9
• S100A10