Seroatlas · Human Serome Atlas

AFAP1

Actin filament-associated protein 1

Also known as: AFAP, AFAP-110, AFAP1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N556
Gene
AFAP1
Ensembl
ENSG00000196526
Chromosome
4
Canonical length
730 aa
Protein class
Predicted intracellular proteins
Subcellular location
Actin filaments,Focal adhesion sites,Cytosol
Quaternary structure
Homomultimer

OverviewNCBI Gene

The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Canonical amino-acid sequenceUniProt

730 residues, UniProt reviewed canonical sequence.

>Q8N556|AFAP1
     1  MEELIVELRL FLELLDHEYL TSTVREKKAV ITNILLRIQS SKGFDVKDHA QKQETANSLP
    61  APPQMPLPEI PQPWLPPDSG PPPLPTSSLP EGYYEEAVPL SPGKAPEYIT SNYDSDAMSS
   121  SYESYDEEEE DGKGKKTRHQ WPSEEASMDL VKDAKICAFL LRKKRFGQWT KLLCVIKDTK
   181  LLCYKSSKDQ QPQMELPLQG CNITYIPKDS KKKKHELKIT QQGTDPLVLA VQSKEQAEQW
   241  LKVIKEAYSG CSGPVDSECP PPPSSPVHKA ELEKKLSSER PSSDGEGVVE NGITTCNGKE
   301  QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT CGYLNVLSNS
   361  RWRERWCRVK DNKLIFHKDR TDLKTHIVSI PLRGCEVIPG LDSKHPLTFR LLRNGQEVAV
   421  LEASSSEDMG RWIGILLAET GSSTDPEALH YDYIDVEMSA SVIQTAKQTF CFMNRRVISA
   481  NPYLGGTSNG YAHPSGTALH YDDVPCINGS LKGKKPPVAS NGVTGKGKTL SSQPKKADPA
   541  AVVKRTGSNA AQYKYGKNRV EADAKRLQTK EEELLKRKEA LRNRLAQLRK ERKDLRAAIE
   601  VNAGRKPQAI LEEKLKQLEE ECRQKEAERV SLELELTEVK ESLKKALAGG VTLGLAIEPK
   661  SGTSSPQSPV FRHRTLENSP ISSCDTSDTE GPVPVNSAAV LKKSQAAPGS SPCRGHVLRK
   721  AKEWELKNGT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AFAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.52
Highest tissue expression
17 nTPM

Expression across tissuesHPA

Tissue

  • colon: 17 nTPM
  • blood vessel: 15 nTPM
  • smooth muscle: 15 nTPM
  • parathyroid gland: 14 nTPM
  • endometrium: 13 nTPM
  • urinary bladder: 13 nTPM

Single-cell type

  • late spermatids: 644 nCPM
  • somatotrophs: 390 nCPM
  • extravillous trophoblasts: 368 nCPM
  • early spermatids: 221 nCPM
  • oligodendrocyte progenitor cells: 204 nCPM
  • epicardial cells: 187 nCPM

Immune cell

  • eosinophil: 2.5 nTPM
  • naive CD4 T-cell: 1 nTPM
  • neutrophil: 0.8 nTPM
  • memory CD4 T-cell: 0.6 nTPM
  • total PBMC: 0.6 nTPM
  • MAIT T-cell: 0.5 nTPM

Brain region

  • amygdala: 18 nTPM
  • cerebral cortex: 16 nTPM
  • hippocampal formation: 15 nTPM
  • basal ganglia: 15 nTPM
  • hypothalamus: 13 nTPM
  • cerebellum: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.67
gnomAD pLI
0
gnomAD missense Z
-0.91
DepMap mean gene effect
0.1
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AFAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AFAP1 as an antibody target. Whether an autoantibody or antibody against AFAP1 could matter depends on whether native AFAP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AFAP1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AFAP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/AFAP1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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