Seroatlas · Human Serome Atlas

ACSF2

Medium-chain acyl-CoA ligase ACSF2, mitochondrial

Also known as: ACSF2_HUMAN, ACSMW, FLJ20920

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96CM8
Gene: ACSF2
Ensembl: ENSG00000167107
Chromosome: 17
Canonical length: 615 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Microtubules,Cytosol

OverviewNCBI Gene

Enables medium-chain fatty acid-CoA ligase activity. Predicted to be involved in fatty acid metabolic process. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

615 residues, UniProt reviewed canonical sequence.

>Q96CM8|ACSF2
     1  MAVYVGMLRL GRLCAGSSGV LGARAALSRS WQEARLQGVR FLSSREVDRM VSTPIGGLSY
    61  VQGCTKKHLN SKTVGQCLET TAQRVPEREA LVVLHEDVRL TFAQLKEEVD KAASGLLSIG
   121  LCKGDRLGMW GPNSYAWVLM QLATAQAGII LVSVNPAYQA MELEYVLKKV GCKALVFPKQ
   181  FKTQQYYNVL KQICPEVENA QPGALKSQRL PDLTTVISVD APLPGTLLLD EVVAAGSTRQ
   241  HLDQLQYNQQ FLSCHDPINI QFTSGTTGSP KGATLSHYNI VNNSNILGER LKLHEKTPEQ
   301  LRMILPNPLY HCLGSVAGTM MCLMYGATLI LASPIFNGKK ALEAISRERG TFLYGTPTMF
   361  VDILNQPDFS SYDISTMCGG VIAGSPAPPE LIRAIINKIN MKDLVVAYGT TENSPVTFAH
   421  FPEDTVEQKA ESVGRIMPHT EARIMNMEAG TLAKLNTPGE LCIRGYCVML GYWGEPQKTE
   481  EAVDQDKWYW TGDVATMNEQ GFCKIVGRSK DMIIRGGENI YPAELEDFFH THPKVQEVQV
   541  VGVKDDRMGE EICACIRLKD GEETTVEEIK AFCKGKISHF KIPKYIVFVT NYPLTISGKI
   601  QKFKLREQME RHLNL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACSF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 133 nTPM

Expression across tissuesHPA

Tissue

kidney: 133 nTPM
adrenal gland: 69 nTPM
thyroid gland: 40 nTPM
skeletal muscle: 40 nTPM
liver: 34 nTPM
colon: 26 nTPM

Single-cell type

proximal tubule cells: 467 nCPM
cytotrophoblasts: 136 nCPM
myonuclei: 116 nCPM
adipocytes: 113 nCPM
enterocytes: 111 nCPM
adrenal cortex cells: 105 nCPM

Immune cell

myeloid DC: 16 nTPM
eosinophil: 15 nTPM
memory B-cell: 8.7 nTPM
intermediate monocyte: 7.7 nTPM
non-classical monocyte: 7 nTPM
plasmacytoid DC: 6.3 nTPM

Brain region

choroid plexus: 9.4 nTPM
medulla oblongata: 9.3 nTPM
basal ganglia: 9 nTPM
spinal cord: 8.7 nTPM
thalamus: 7.9 nTPM
cerebellum: 7.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.65
gnomAD pLI: 0
gnomAD missense Z: 1.98
DepMap mean gene effect: -0.15
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACSF2 as an antibody target. Whether an autoantibody or antibody against ACSF2 could matter depends on whether native ACSF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACSF2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACSF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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