Seroatlas · Human Serome Atlas

ZNF528

Zinc finger protein 528

Also known as: KIAA1827, ZN528_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q3MIS6
Gene: ZNF528
Ensembl: ENSG00000167555
Chromosome: 19
Canonical length: 628 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nuclear speckles,Cytosol

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

628 residues, UniProt reviewed canonical sequence.

>Q3MIS6|ZNF528
     1  MALTQGPLKF MDVAIEFSQE EWKCLDPAQR TLYRDVMLEN YRNLVSLGIC LPDLSVTSML
    61  EQKRDPWTLQ SEEKIANDPD GRECIKGVNT ERSSKLGSNA GNKPCKNQLG FTFQLHLSDL
   121  QLFQAERKIS GCKHFEKPVS DNSSVSPLEK ISSSVKSHLL NKYRNNFDHA PLLPQEQKAH
   181  IREKAYKCNE HGQVFRASAS LTNQVIHNAD NPYKCSECGK VFSCSSKLVI HRRMHTGEKP
   241  YKCHECGKLF SSNSNLSQHQ RIHTGEKPYK CHECDKVFRS SSKLAQHQRI HTGEKPYKCH
   301  ECDKVFNQIA HLVRHQKIHT GEKPYSCNKC GKVFSRHSYL AEHQTVHTGE KPYKCEECGK
   361  AFSVRSSLIT HQLIHTGRKP YKCKECDKVF GRKCFLTSHQ RIHTRERPYG CSQCGKIFSQ
   421  KSDLIRHRKT HTDEKPYKCN KCGTAFREFS DLTAHFLIHS GEKPYECKEC GKVFRYKSSL
   481  TSHHRIHTGE KPYKCNRCGK VFSRSSNLVC HQKIHTGEKP YKCNQCGKVF NQASYLTRHQ
   541  IIHTGERPYR CSKCGKAFRG CSGLTAHLAI HTEKKSHECK ECGKIFTQKS SLTNHHRIHI
   601  GEKPYKCTLC SKVFSHNSDL AQHQRVHS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF528 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 18 nTPM

Expression across tissuesHPA

Tissue

retina: 18 nTPM
epididymis: 18 nTPM
thyroid gland: 17 nTPM
cerebellum: 14 nTPM
ovary: 13 nTPM
fallopian tube: 13 nTPM

Single-cell type

thyrotrophs: 53 nCPM
pituitary stem cells: 45 nCPM
pituicytes/fscs: 44 nCPM
bergmann glia: 38 nCPM
lactotrophs: 35 nCPM
tuft cells: 35 nCPM

Immune cell

NK-cell: 18 nTPM
MAIT T-cell: 13 nTPM
eosinophil: 12 nTPM
naive B-cell: 11 nTPM
memory CD8 T-cell: 10 nTPM
T-reg: 9.6 nTPM

Brain region

cerebellum: 76 nTPM
white matter: 67 nTPM
pons: 64 nTPM
hypothalamus: 62 nTPM
basal ganglia: 62 nTPM
choroid plexus: 61 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.67
gnomAD pLI: 0
gnomAD missense Z: -1.36
DepMap mean gene effect: 0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF528 as an antibody target. Whether an autoantibody or antibody against ZNF528 could matter depends on whether native ZNF528 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF528 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF528 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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