Seroatlas · Human Serome Atlas

ZNF396

Zinc finger protein 396

Also known as: FLJ31213, ZN396_HUMAN, ZSCAN14

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96N95
Gene: ZNF396
Ensembl: ENSG00000186496
Chromosome: 18
Canonical length: 335 aa
Protein class: Predicted intracellular proteins, Transcription factors

OverviewNCBI Gene

Enables protein heterodimerization activity; protein homodimerization activity; and sequence-specific double-stranded DNA binding activity. Involved in negative regulation of DNA-templated transcription. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

335 residues, UniProt reviewed canonical sequence.

>Q96N95|ZNF396
     1  MSAKLGKSSS LLTQTSEECN GILTEKMEEE EQTCDPDSSL HWSSSYSPET FRQQFRQFGY
    61  QDSPGPHEAL SRLWELCHLW LRPEVHTKEQ ILELLVLEQF LAILPKELQA WVQKHHPENG
   121  EETVTMLEDV ERELDGPKQI FFGRRKDMIA EKLAPSEITE ELPSSQLMPV KKQLQGASWE
   181  LQSLRPHDED IKTTNVKSAS RQKTSLGIEL HCNVSNILHM NGSQSSTYRG TYEQDGRFEK
   241  RQGNPSWKKQ QKCDECGKIF SQSSALILHQ RIHSGKKPYA CDECAKAFSR SAILIQHRRT
   301  HTGEKPYKCH DCGKAFSQSS NLFRHRKRHI RKKVP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF396 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.55
Highest tissue expression: 5.1 nTPM

Expression across tissuesHPA

Tissue

thyroid gland: 5.1 nTPM
choroid plexus: 4.7 nTPM
testis: 4.7 nTPM
fallopian tube: 4.6 nTPM
parathyroid gland: 4.1 nTPM
retina: 3.6 nTPM

Single-cell type

respiratory ciliated cells: 21 nCPM
fallopian tube ciliated cells: 21 nCPM
early spermatids: 18 nCPM
late spermatids: 15 nCPM
late primary spermatocytes: 14 nCPM
neutrophils: 14 nCPM

Immune cell

basophil: 8.8 nTPM
neutrophil: 5.1 nTPM
eosinophil: 2.5 nTPM
naive CD4 T-cell: 1.6 nTPM
gdT-cell: 1.5 nTPM
NK-cell: 1.4 nTPM

Brain region

choroid plexus: 16 nTPM
cerebellum: 13 nTPM
midbrain: 13 nTPM
medulla oblongata: 13 nTPM
white matter: 12 nTPM
basal ganglia: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.12
gnomAD pLI: 0
gnomAD missense Z: 0.49
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF396 as an antibody target. Whether an autoantibody or antibody against ZNF396 could matter depends on whether native ZNF396 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF396 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF396 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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