Seroatlas · Human Serome Atlas

ZNF281

Zinc finger protein 281

Also known as: ZBP-99, ZN281_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y2X9
Gene: ZNF281
Ensembl: ENSG00000162702
Chromosome: 1
Canonical length: 895 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of gene expression; negative regulation of transcription by RNA polymerase II; and positive regulation of DNA-templated transcription. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

895 residues, UniProt reviewed canonical sequence.

>Q9Y2X9|ZNF281
     1  MKIGSGFLSG GGGTGSSGGS GSGGGGSGGG GGGGSSGRRA EMEPTFPQGM VMFNHRLPPV
    61  TSFTRPAGSA APPPQCVLSS STSAAPAAEP PPPPAPDMTF KKEPAASAAA FPSQRTSWGF
   121  LQSLVSIKQE KPADPEEQQS HHHHHHHHYG GLFAGAEERS PGLGGGEGGS HGVIQDLSIL
   181  HQHVQQQPAQ HHRDVLLSSS SRTDDHHGTE EPKQDTNVKK AKRPKPESQG IKAKRKPSAS
   241  SKPSLVGDGE GAILSPSQKP HICDHCSAAF RSSYHLRRHV LIHTGERPFQ CSQCSMGFIQ
   301  KYLLQRHEKI HSREKPFGCD QCSMKFIQKY HMERHKRTHS GEKPYKCDTC QQYFSRTDRL
   361  LKHRRTCGEV IVKGATSAEP GSSNHTNMGN LAVLSQGNTS SSRRKTKSKS IAIENKEQKT
   421  GKTNESQISN NINMQSYSVE MPTVSSSGGI IGTGIDELQK RVPKLIFKKG SRKNTDKNYL
   481  NFVSPLPDIV GQKSLSGKPS GSLGIVSNNS VETIGLLQST SGKQGQISSN YDDAMQFSKK
   541  RRYLPTASSN SAFSINVGHM VSQQSVIQSA GVSVLDNEAP LSLIDSSALN AEIKSCHDKS
   601  GIPDEVLQSI LDQYSNKSES QKEDPFNIAE PRVDLHTSGE HSELVQEENL SPGTQTPSND
   661  KASMLQEYSK YLQQAFEKST NASFTLGHGF QFVSLSSPLH NHTLFPEKQI YTTSPLECGF
   721  GQSVTSVLPS SLPKPPFGML FGSQPGLYLS ALDATHQQLT PSQELDDLID SQKNLETSSA
   781  FQSSSQKLTS QKEQKNLESS TGFQIPSQEL ASQIDPQKDI EPRTTYQIEN FAQAFGSQFK
   841  SGSRVPMTFI TNSNGEVDHR VRTSVSDFSG YTNMMSDVSE PCSTRVKTPT SQSYR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF281 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.67
Highest tissue expression: 32 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 32 nTPM
liver: 20 nTPM
thymus: 12 nTPM
tonsil: 11 nTPM
lymph node: 11 nTPM
appendix: 10 nTPM

Single-cell type

neutrophils: 385 nCPM
hepatocytes: 96 nCPM
neutrophil progenitors: 94 nCPM
monocytes: 80 nCPM
breast hormone-responsive cells: 72 nCPM
oligodendrocytes: 53 nCPM

Immune cell

neutrophil: 38 nTPM
eosinophil: 30 nTPM
basophil: 15 nTPM
naive CD4 T-cell: 14 nTPM
naive CD8 T-cell: 12 nTPM
T-reg: 11 nTPM

Brain region

white matter: 45 nTPM
basal ganglia: 28 nTPM
cerebellum: 26 nTPM
cerebral cortex: 23 nTPM
medulla oblongata: 22 nTPM
midbrain: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.25
gnomAD pLI: 1
gnomAD missense Z: 1.02
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF281 as an antibody target. Whether an autoantibody or antibody against ZNF281 could matter depends on whether native ZNF281 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF281 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF281 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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