Seroatlas · Human Serome Atlas

USP50

Ubiquitin carboxyl-terminal hydrolase 50

Also known as: UBP50_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q70EL3
Gene: USP50
Ensembl: ENSG00000170236
Chromosome: 15
Canonical length: 339 aa
Protein class: Enzymes, Predicted intracellular proteins

OverviewNCBI Gene

Enables cysteine-type deubiquitinase activity. Involved in regulation of cell cycle G2/M phase transition. Acts upstream of or within several processes, including nuclear speck organization; positive regulation of NLRP3 inflammasome complex assembly; and positive regulation of interleukin-1 beta production. Located in centrosome; cytoplasm; and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

339 residues, UniProt reviewed canonical sequence.

>Q70EL3|USP50
     1  MTSQPSLPAD DFDIYHVLAE CTDYYDTLPV KEADGNQPHF QGVTGLWNLG NTCCVNAISQ
    61  CLCSILPLVE YFLTGKYITA LQKFLLPSDC SEVATAFAYL MTDMWLGDSD CVSPEIFWSA
   121  LGNLYPAFTK KMQQDAQEFL ICVLNELHEA LKKYHYSRRR SYEKGSTQRC CRKWITTETS
   181  IITQLFEEQL NYSIVCLKCE KCTYKNEVFT VFSLPIPSKY ECSLRDCLQC FFQQDALTWN
   241  NEIHCSFCET KQETAVRASI SKAPKIIIFH LKRFDIQGTT KRKLRTDIHY PLTNLDLTPY
   301  ICSIFRKYPK YNLCAVVNHF GDLDGGHYTA FCKNSVTQA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USP50 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 1.3 nTPM

Expression across tissuesHPA

Tissue

testis: 1.3 nTPM
kidney: 0.2 nTPM
pituitary gland: 0.2 nTPM
basal ganglia: 0.1 nTPM
cerebellum: 0.1 nTPM
cerebral cortex: 0.1 nTPM

Single-cell type

epicardial cells: 196 nCPM
late primary spermatocytes: 173 nCPM
oocytes: 157 nCPM
early spermatids: 156 nCPM
late spermatids: 126 nCPM
cardiomyocytes: 105 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 0.4 nTPM
basal ganglia: 0.3 nTPM
cerebral cortex: 0.3 nTPM
medulla oblongata: 0.3 nTPM
pons: 0.3 nTPM
spinal cord: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.87
gnomAD pLI: 0
gnomAD missense Z: 0.39
DepMap mean gene effect: -0.09
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads USP50 as an antibody target. Whether an autoantibody or antibody against USP50 could matter depends on whether native USP50 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

USP50 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label USP50 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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