USP40

Protein identityUniProt · HPA

UniProt accession

Q9NVE5

Gene

USP40

Ensembl

ENSG00000085982

Chromosome

2

Canonical length

1235 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Vesicles,Focal adhesion sites

OverviewNCBI Gene

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP40 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

1235 residues, UniProt reviewed canonical sequence.

>Q9NVE5|USP40
     1  MFGDLFEEEY STVSNNQYGK GKKLKTKALE PPAPREFTNL SGIRNQGGTC YLNSLLQTLH
    61  FTPEFREALF SLGPEELGLF EDKDKPDAKV RIIPLQLQRL FAQLLLLDQE AASTADLTDS
   121  FGWTSNEEMR QHDVQELNRI LFSALETSLV GTSGHDLIYR LYHGTIVNQI VCKECKNVSE
   181  RQEDFLDLTV AVKNVSGLED ALWNMYVEEE VFDCDNLYHC GTCDRLVKAA KSAKLRKLPP
   241  FLTVSLLRFN FDFVKCERYK ETSCYTFPLR INLKPFCEQS ELDDLEYIYD LFSVIIHKGG
   301  CYGGHYHVYI KDVDHLGNWQ FQEEKSKPDV NLKDLQSEEE IDHPLMILKA ILLEENNLIP
   361  VDQLGQKLLK KIGISWNKKY RKQHGPLRKF LQLHSQIFLL SSDESTVRLL KNSSLQAESD
   421  FQRNDQQIFK MLPPESPGLN NSISCPHWFD INDSKVQPIR EKDIEQQFQG KESAYMLFYR
   481  KSQLQRPPEA RANPRYGVPC HLLNEMDAAN IELQTKRAEC DSANNTFELH LHLGPQYHFF
   541  NGALHPVVSQ TESVWDLTFD KRKTLGDLRQ SIFQLLEFWE GDMVLSVAKL VPAGLHIYQS
   601  LGGDELTLCE TEIADGEDIF VWNGVEVGGV HIQTGIDCEP LLLNVLHLDT SSDGEKCCQV
   661  IESPHVFPAN AEVGTVLTAL AIPAGVIFIN SAGCPGGEGW TAIPKEDMRK TFREQGLRNG
   721  SSILIQDSHD DNSLLTKEEK WVTSMNEIDW LHVKNLCQLE SEEKQVKISA TVNTMVFDIR
   781  IKAIKELKLM KELADNSCLR PIDRNGKLLC PVPDSYTLKE AELKMGSSLG LCLGKAPSSS
   841  QLFLFFAMGS DVQPGTEMEI VVEETISVRD CLKLMLKKSG LQGDAWHLRK MDWCYEAGEP
   901  LCEEDATLKE LLICSGDTLL LIEGQLPPLG FLKVPIWWYQ LQGPSGHWES HQDQTNCTSS
   961  WGRVWRATSS QGASGNEPAQ VSLLYLGDIE ISEDATLAEL KSQAMTLPPF LEFGVPSPAH
  1021  LRAWTVERKR PGRLLRTDRQ PLREYKLGRR IEICLEPLQK GENLGPQDVL LRTQVRIPGE
  1081  RTYAPALDLV WNAAQGGTAG SLRQRVADFY RLPVEKIEIA KYFPEKFEWL PISSWNQQIT
  1141  KRKKKKKQDY LQGAPYYLKD GDTIGVKNLL IDDDDDFSTI RDDTGKEKQK QRALGRRKSQ
  1201  EALHEQSSYI LSSAETPARP RAPETSLSIH VGSFR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USP40 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

22 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about USP40.

Disease | ImmuneIEDB

Conditions an epitope on USP40 was assayed in.

• lung adenocarcinoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.93

gnomAD pLI

0

gnomAD missense Z

0.24

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of apoptotic process
• protein deubiquitination
• proteolysis
• regulation of protein stability

Molecular functions

• cysteine-type deubiquitinase activity

Cellular components

• cytoplasm
• cytosol
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Peptidase C19, ubiquitin carboxyl-terminal hydrolase
• Ubiquitin specific protease, conserved site
• Ubiquitin specific protease UPS, catalytic domain
• Papain-like cysteine peptidase superfamily
• Ubiquitin carboxyl-terminal hydrolases
• Ubiquitin carboxyl-terminal hydrolase
• Ubiquitin carboxyl-terminal hydrolase 40, ubiquitin-like domain
• USP40 ubiquitin-like domain

KeywordsUniProt

• Cytoplasm
• Hydrolase
• Protease
• Thiol protease
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of USP40 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GMEB1