Seroatlas · Human Serome Atlas

USP35

Ubiquitin carboxyl-terminal hydrolase 35

Also known as: KIAA1372, UBP35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9P2H5
Gene: USP35
Ensembl: ENSG00000118369
Chromosome: 11
Canonical length: 1018 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli fibrillar center
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a member of the peptidase C19 family of ubiquitin-specific proteases. These deubiquitinating enzymes (DUBs) catalyze the removal of ubiquitin proteins from other proteins. The encoded protein associates with polarized mitochondria and has been shown to inhibit NF-kappa B activation and delay PARK2-mediated degradation of mitochondria. Expression of this gene is upregulated by the let-7a microRNA and reduced expression has been observed in human tumor tissues. [provided by RefSeq, Jul 2017]

Canonical amino-acid sequenceUniProt

1018 residues, UniProt reviewed canonical sequence.

>Q9P2H5|USP35
     1  MDKILEAVVT SSYPVSVKQG LVRRVLEAAR QPLEREQCLA LLALGARLYV GGAEELPRRV
    61  GCQLLHVAGR HHPDVFAEFF SARRVLRLLQ GGAGPPGPRA LACVQLGLQL LPEGPAADEV
   121  FALLRREVLR TVCERPGPAA CAQVARLLAR HPRCVPDGPH RLLFCQQLVR CLGRFRCPAE
   181  GEEGAVEFLE QAQQVSGLLA QLWRAQPAAI LPCLKELFAV ISCAEEEPPS SALASVVQHL
   241  PLELMDGVVR NLSNDDSVTD SQMLTAISRM IDWVSWPLGK NIDKWIIALL KGLAAVKKFS
   301  ILIEVSLTKI EKVFSKLLYP IVRGAALSVL KYMLLTFQHS HEAFHLLLPH IPPMVASLVK
   361  EDSNSGTSCL EQLAELVHCM VFRFPGFPDL YEPVMEAIKD LHVPNEDRIK QLLGQDAWTS
   421  QKSELAGFYP RLMAKSDTGK IGLINLGNTC YVNSILQALF MASDFRHCVL RLTENNSQPL
   481  MTKLQWLFGF LEHSQRPAIS PENFLSASWT PWFSPGTQQD CSEYLKYLLD RLHEEEKTGT
   541  RICQKLKQSS SPSPPEEPPA PSSTSVEKMF GGKIVTRICC LCCLNVSSRE EAFTDLSLAF
   601  PPPERCRRRR LGSVMRPTED ITARELPPPT SAQGPGRVGP RRQRKHCITE DTPPTSLYIE
   661  GLDSKEAGGQ SSQEERIERE EEGKEERTEK EEVGEEEEST RGEGEREKEE EVEEEEEKVE
   721  KETEKEAEQE KEEDSLGAGT HPDAAIPSGE RTCGSEGSRS VLDLVNYFLS PEKLTAENRY
   781  YCESCASLQD AEKVVELSQG PCYLILTLLR FSFDLRTMRR RKILDDVSIP LLLRLPLAGG
   841  RGQAYDLCSV VVHSGVSSES GHYYCYAREG AARPAASLGT ADRPEPENQW YLFNDTRVSF
   901  SSFESVSNVT SFFPKDTAYV LFYRQRPREG PEAELGSSRV RTEPTLHKDL MEAISKDNIL
   961  YLQEQEKEAR SRAAYISALP TSPHWGRGFD EDKDEDEGSP GGCNPAGGNG GDFHRLVF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USP35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 22 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 22 nTPM
adrenal gland: 18 nTPM
liver: 16 nTPM
pituitary gland: 12 nTPM
parathyroid gland: 12 nTPM
heart muscle: 9.5 nTPM

Single-cell type

late spermatids: 146 nCPM
early spermatids: 55 nCPM
gonadotrophs: 25 nCPM
epicardial cells: 16 nCPM
lactotrophs: 14 nCPM
astrocytes: 13 nCPM

Immune cell

eosinophil: 0.8 nTPM
intermediate monocyte: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
gdT-cell: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

pons: 27 nTPM
midbrain: 25 nTPM
thalamus: 24 nTPM
cerebral cortex: 24 nTPM
amygdala: 23 nTPM
basal ganglia: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.72
gnomAD pLI: 0
gnomAD missense Z: -0.29
DepMap mean gene effect: -0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

cysteine-type deubiquitinase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of USP35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

HSP90AA1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads USP35 as an antibody target. Whether an autoantibody or antibody against USP35 could matter depends on whether native USP35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

USP35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label USP35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...