Seroatlas · Human Serome Atlas

TTK

Dual specificity protein kinase TTK

Also known as: CT96, MPH1, MPS1, MPS1L1, TTK_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P33981
Gene
TTK
Ensembl
ENSG00000112742
Chromosome
6
Canonical length
857 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoli,Cytosol

OverviewNCBI Gene

This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]

Canonical amino-acid sequenceUniProt

857 residues, UniProt reviewed canonical sequence.

>P33981|TTK
     1  MESEDLSGRE LTIDSIMNKV RDIKNKFKNE DLTDELSLNK ISADTTDNSG TVNQIMMMAN
    61  NPEDWLSLLL KLEKNSVPLS DALLNKLIGR YSQAIEALPP DKYGQNESFA RIQVRFAELK
   121  AIQEPDDARD YFQMARANCK KFAFVHISFA QFELSQGNVK KSKQLLQKAV ERGAVPLEML
   181  EIALRNLNLQ KKQLLSEEEK KNLSASTVLT AQESFSGSLG HLQNRNNSCD SRGQTTKARF
   241  LYGENMPPQD AEIGYRNSLR QTNKTKQSCP FGRVPVNLLN SPDCDVKTDD SVVPCFMKRQ
   301  TSRSECRDLV VPGSKPSGND SCELRNLKSV QNSHFKEPLV SDEKSSELII TDSITLKNKT
   361  ESSLLAKLEE TKEYQEPEVP ESNQKQWQSK RKSECINQNP AASSNHWQIP ELARKVNTEQ
   421  KHTTFEQPVF SVSKQSPPIS TSKWFDPKSI CKTPSSNTLD DYMSCFRTPV VKNDFPPACQ
   481  LSTPYGQPAC FQQQQHQILA TPLQNLQVLA SSSANECISV KGRIYSILKQ IGSGGSSKVF
   541  QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM
   601  VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML
   661  KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL
   721  GCILYYMTYG KTPFQQIINQ ISKLHAIIDP NHEIEFPDIP EKDLQDVLKC CLKRDPKQRI
   781  SIPELLAHPY VQIQTHPVNQ MAKGTTEEMK YVLGQLVGLN SPNSILKAAK TLYEHYSGGE
   841  SHNSSSSKTF EKKRGKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TTK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.48
Highest tissue expression
43 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 43 nTPM
  • testis: 32 nTPM
  • bone marrow: 24 nTPM
  • tonsil: 8.4 nTPM
  • lymph node: 7.6 nTPM
  • rectum: 5.5 nTPM

Single-cell type

  • megakaryocytes: 661 nCPM
  • late primary spermatocytes: 229 nCPM
  • oocytes: 120 nCPM
  • monocyte progenitors: 79 nCPM
  • megakaryocyte progenitors: 55 nCPM
  • erythrocyte progenitors: 54 nCPM

Immune cell

  • T-reg: 1.2 nTPM
  • naive B-cell: 0.7 nTPM
  • memory CD8 T-cell: 0.4 nTPM
  • eosinophil: 0.3 nTPM
  • NK-cell: 0.3 nTPM
  • memory B-cell: 0.2 nTPM

Brain region

  • cerebral cortex: 0.7 nTPM
  • pons: 0.6 nTPM
  • thalamus: 0.6 nTPM
  • white matter: 0.6 nTPM
  • medulla oblongata: 0.4 nTPM
  • cerebellum: 0.3 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TTK.

Disease | ImmuneIEDB

Conditions an epitope on TTK was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.46
gnomAD pLI
0
gnomAD missense Z
0.72
DepMap mean gene effect
-0.87
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TTK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TTK as an antibody target. Whether an autoantibody or antibody against TTK could matter depends on whether native TTK is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TTK is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TTK as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TTK. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...