Seroatlas · Human Serome Atlas

TSPYL5

Testis-specific Y-encoded-like protein 5

Also known as: KIAA1750, TSYL5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86VY4
Gene: TSPYL5
Ensembl: ENSG00000180543
Chromosome: 8
Canonical length: 417 aa
Protein class: Predicted intracellular proteins
Subcellular location: Golgi apparatus,Cytosol

OverviewNCBI Gene

Predicted to enable chromatin binding activity and histone binding activity. Involved in several processes, including cellular response to gamma radiation; positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; and positive regulation of protein ubiquitination. Predicted to be active in chromatin and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

417 residues, UniProt reviewed canonical sequence.

>Q86VY4|TSPYL5
     1  MSGRSRGRKS SRAKNRGKGR AKARVRPAPD DAPRDPDPSQ YQSLGEDTQA AQVQAGAGWG
    61  GLEAAASAQL LRLGEEAACR LPLDCGLALR ARAAGDHGQA AARPGPGKAA SLSERLAADT
   121  VFVGTAGTVG RPKNAPRVGN RRGPAGKKAP ETCSTAGRGP QVIAGGRQKK GAAGENTSVS
   181  AGEEKKEERD AGSGPPATEG SMDTLENVQL KLENMNAQAD RAYLRLSRKF GQLRLQHLER
   241  RNHLIQNIPG FWGQAFQNHP QLASFLNSQE KEVLSYLNSL EVEELGLARL GYKIKFYFDR
   301  NPYFQNKVLI KEYGCGPSGQ VVSRSTPIQW LPGHDLQSLS QGNPENNRSF FGWFSNHSSI
   361  ESDKIVEIIN EELWPNPLQF YLLSEGARVE KGKEKEGRQG PGKQPMETTQ PGVSQSN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPYL5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.55
Highest tissue expression: 42 nTPM

Expression across tissuesHPA

Tissue

testis: 42 nTPM
ovary: 20 nTPM
seminal vesicle: 17 nTPM
epididymis: 15 nTPM
basal ganglia: 15 nTPM
hypothalamus: 14 nTPM

Single-cell type

late spermatids: 783 nCPM
early spermatids: 212 nCPM
late primary spermatocytes: 61 nCPM
undifferentiated spermatogonia: 52 nCPM
pancreatic islet cells: 30 nCPM
corticotrophs: 29 nCPM

Immune cell

myeloid DC: 0.5 nTPM
plasmacytoid DC: 0.5 nTPM
naive B-cell: 0.3 nTPM
naive CD4 T-cell: 0.2 nTPM
NK-cell: 0.2 nTPM
T-reg: 0.2 nTPM

Brain region

hypothalamus: 45 nTPM
pons: 35 nTPM
basal ganglia: 34 nTPM
cerebral cortex: 31 nTPM
medulla oblongata: 29 nTPM
hippocampal formation: 28 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.45
gnomAD pLI: 0.85
gnomAD missense Z: 1.22
DepMap mean gene effect: -0.29
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Growth regulation

InteractionsUniProt · HPA

Protein binding partners of TSPYL5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

USP7

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPYL5 as an antibody target. Whether an autoantibody or antibody against TSPYL5 could matter depends on whether native TSPYL5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPYL5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TSPYL5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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