Seroatlas · Human Serome Atlas

TSPAN8

Tetraspanin-8

Also known as: CO-029, TM4SF3, TSN8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P19075
Gene: TSPAN8
Ensembl: ENSG00000127324
Chromosome: 12
Canonical length: 237 aa
Protein class: Cancer-related genes, Predicted membrane proteins
Subcellular location: Vesicles
Quaternary structure: Homooligomer

OverviewNCBI Gene

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

237 residues, UniProt reviewed canonical sequence.

>P19075|TSPAN8
     1  MAGVSACIKY SMFTFNFLFW LCGILILALA IWVRVSNDSQ AIFGSEDVGS SSYVAVDILI
    61  AVGAIIMILG FLGCCGAIKE SRCMLLLFFI GLLLILLLQV ATGILGAVFK SKSDRIVNET
   121  LYENTKLLSA TGESEKQFQE AIIVFQEEFK CCGLVNGAAD WGNNFQHYPE LCACLDKQRP
   181  CQSYNGKQVY KETCISFIKD FLAKNLIIVI GISFGLAVIE ILGLVFSMVL YCQIGNK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPAN8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 4
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 892 nTPM

Expression across tissuesHPA

Tissue

colon: 892 nTPM
duodenum: 862 nTPM
rectum: 809 nTPM
small intestine: 799 nTPM
stomach: 596 nTPM
gallbladder: 196 nTPM

Single-cell type

enterocytes: 3,175 nCPM
colonocytes: 2,747 nCPM
enteric stem cells: 2,166 nCPM
enteric transient amplifying cells: 1,562 nCPM
gastric progenitor cells: 1,533 nCPM
paneth cells: 1,493 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 28 nTPM
medulla oblongata: 25 nTPM
spinal cord: 16 nTPM
pons: 14 nTPM
cerebellum: 11 nTPM
basal ganglia: 8.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.84
gnomAD pLI: 0.01
gnomAD missense Z: 0.35
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

integrin binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TSPAN8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPAN8 as an antibody target. Whether an autoantibody or antibody against TSPAN8 could matter depends on whether native TSPAN8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPAN8 is annotated at the cell surface, where native TSPAN8 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TSPAN8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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