Seroatlas · Human Serome Atlas

TSPAN4

Tetraspanin-4

Also known as: NAG-2, TETRASPAN, TM4SF7, TSN4_HUMAN, TSPAN-4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O14817
Gene
TSPAN4
Ensembl
ENSG00000214063
Chromosome
11
Canonical length
238 aa
Protein class
Predicted membrane proteins

OverviewNCBI Gene

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

238 residues, UniProt reviewed canonical sequence.

>O14817|TSPAN4
     1  MARACLQAVK YLMFAFNLLF WLGGCGVLGV GIWLAATQGS FATLSSSFPS LSAANLLIIT
    61  GAFVMAIGFV GCLGAIKENK CLLLTFFLLL LLVFLLEATI AILFFAYTDK IDRYAQQDLK
   121  KGLHLYGTQG NVGLTNAWSI IQTDFRCCGV SNYTDWFEVY NATRVPDSCC LEFSESCGLH
   181  APGTWWKAPC YETVKVWLQE NLLAVGIFGL CTALVQILGL TFAMTMYCQV VKADTYCA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPAN4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
122 nTPM

Expression across tissuesHPA

Tissue

  • adipose tissue: 122 nTPM
  • breast: 98 nTPM
  • lung: 98 nTPM
  • ovary: 86 nTPM
  • endometrium: 81 nTPM
  • spleen: 80 nTPM

Single-cell type

  • hofbauer cells: 366 nCPM
  • decidual stromal cells: 268 nCPM
  • melanocytes: 175 nCPM
  • kupffer cells: 173 nCPM
  • alveolar cells type 1: 156 nCPM
  • lymphatic endothelial cells: 148 nCPM

Immune cell

  • classical monocyte: 147 nTPM
  • intermediate monocyte: 144 nTPM
  • myeloid DC: 101 nTPM
  • NK-cell: 96 nTPM
  • total PBMC: 65 nTPM
  • non-classical monocyte: 40 nTPM

Brain region

  • choroid plexus: 73 nTPM
  • hypothalamus: 21 nTPM
  • medulla oblongata: 19 nTPM
  • cerebellum: 18 nTPM
  • midbrain: 18 nTPM
  • amygdala: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.41
gnomAD pLI
0
gnomAD missense Z
-0.4
DepMap mean gene effect
-0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TSPAN4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPAN4 as an antibody target. Whether an autoantibody or antibody against TSPAN4 could matter depends on whether native TSPAN4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPAN4 is annotated at the cell surface, where native TSPAN4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TSPAN4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TSPAN4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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