Seroatlas · Human Serome Atlas

TRIM61

Putative tripartite motif-containing protein 61

Also known as: RNF35, TRI61_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5EBN2
Gene: TRIM61
Ensembl: ENSG00000183439
Chromosome: 4
Canonical length: 209 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli fibrillar center,Endoplasmic reticulum

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in innate immune response and regulation of gene expression. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

209 residues, UniProt reviewed canonical sequence.

>Q5EBN2|TRIM61
     1  MEFVTALADL RAEASCPICL DYLKDPVTIS CGHNFCLSCI IMSWKDLHDS FPCPFCHFCC
    61  PERKFISNPQ LGSLTEIAKQ LQIRSKKRKR QEEKHVCKKH NQVLTFFCQK DLELLCPRCS
   121  LSTDHQHHCV WPIKKAASYH RKKLEEYNAP WKERVELIEK VITMQTRKSL ELKKKMESPS
   181  VTRLECSCTI SAHFNLRLPG SSDSSASGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRIM61 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 8.3 nTPM

Expression across tissuesHPA

Tissue

testis: 8.3 nTPM
thyroid gland: 5.3 nTPM
ovary: 5 nTPM
bone marrow: 3.5 nTPM
adrenal gland: 3.4 nTPM
fallopian tube: 3.3 nTPM

Single-cell type

late primary spermatocytes: 143 nCPM
cardiomyocytes: 78 nCPM
adipocytes: 27 nCPM
early spermatids: 23 nCPM
oocytes: 23 nCPM
early primary spermatocytes: 13 nCPM

Immune cell

NK-cell: 8.1 nTPM
naive B-cell: 5.8 nTPM
basophil: 5.4 nTPM
naive CD4 T-cell: 4.4 nTPM
plasmacytoid DC: 4.1 nTPM
naive CD8 T-cell: 3.7 nTPM

Brain region

hypothalamus: 13 nTPM
midbrain: 12 nTPM
white matter: 12 nTPM
medulla oblongata: 12 nTPM
thalamus: 11 nTPM
pons: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.76
gnomAD pLI: 0
gnomAD missense Z: 1.04
DepMap mean gene effect: -0.31
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

cytoplasm

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRIM61 as an antibody target. Whether an autoantibody or antibody against TRIM61 could matter depends on whether native TRIM61 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRIM61 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRIM61 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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