Seroatlas · Human Serome Atlas

TRAM2

Translocating chain-associated membrane protein 2

Also known as: KIAA0057, TRAM2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q15035
Gene: TRAM2
Ensembl: ENSG00000065308
Chromosome: 6
Canonical length: 370 aa
Protein class: Predicted membrane proteins, Transporters
Subcellular location: Cytosol

OverviewNCBI Gene

TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane.[supplied by OMIM, Jul 2004]

Canonical amino-acid sequenceUniProt

370 residues, UniProt reviewed canonical sequence.

>Q15035|TRAM2
     1  MAFRRRTKSY PLFSQEFVIH NHADIGFCLV LCVLIGLMFE VTAKTAFLFI LPQYNISVPT
    61  ADSETVHYHY GPKDLVTILF YIFITIILHA VVQEYILDKI SKRLHLSKVK HSKFNESGQL
   121  VVFHFTSVIW CFYVVVTEGY LTNPRSLWED YPHVHLPFQV KFFYLCQLAY WLHALPELYF
   181  QKVRKEEIPR QLQYICLYLV HIAGAYLLNL SRLGLILLLL QYSTEFLFHT ARLFYFADEN
   241  NEKLFSAWAA VFGVTRLFIL TLAVLAIGFG LARMENQAFD PEKGNFNTLF CRLCVLLLVC
   301  AAQAWLMWRF IHSQLRHWRE YWNEQSAKRR VPATPRLPAR LIKRESGYHE NGVVKAENGT
   361  SPRTKKLKSP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRAM2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 8
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 34 nTPM

Expression across tissuesHPA

Tissue

placenta: 34 nTPM
adipose tissue: 18 nTPM
spleen: 18 nTPM
blood vessel: 18 nTPM
smooth muscle: 16 nTPM
gallbladder: 16 nTPM

Single-cell type

podocytes: 210 nCPM
late spermatids: 199 nCPM
plasma cells: 143 nCPM
hepatocytes: 89 nCPM
oocytes: 83 nCPM
tuft cells: 81 nCPM

Immune cell

non-classical monocyte: 2.1 nTPM
memory B-cell: 1.2 nTPM
intermediate monocyte: 1.1 nTPM
myeloid DC: 1.1 nTPM
classical monocyte: 1 nTPM
eosinophil: 1 nTPM

Brain region

choroid plexus: 15 nTPM
cerebral cortex: 8.3 nTPM
thalamus: 8.1 nTPM
medulla oblongata: 7.7 nTPM
basal ganglia: 7.3 nTPM
pons: 6.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.35
gnomAD pLI: 0.94
gnomAD missense Z: 1.76
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TRAM2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

COL1A1
SERCA2B
ATP2A2

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRAM2 as an antibody target. Whether an autoantibody or antibody against TRAM2 could matter depends on whether native TRAM2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRAM2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRAM2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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