Seroatlas · Human Serome Atlas

TMEM25

Transmembrane protein 25

Also known as: FLJ14399, TMM25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86YD3
Gene: TMEM25
Ensembl: ENSG00000149582
Chromosome: 11
Canonical length: 366 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Golgi apparatus,Vesicles
Secretome location: Intracellular and membrane

OverviewNCBI Gene

Predicted to be involved in negative regulation of excitatory postsynaptic potential and regulation of protein stability. Predicted to be located in late endosome and lysosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

366 residues, UniProt reviewed canonical sequence.

>Q86YD3|TMEM25
     1  MALPPGPAAL RHTLLLLPAL LSSGWGELEP QIDGQTWAER ALRENERHAF TCRVAGGPGT
    61  PRLAWYLDGQ LQEASTSRLL SVGGEAFSGG TSTFTVTAHR AQHELNCSLQ DPRSGRSANA
   121  SVILNVQFKP EIAQVGAKYQ EAQGPGLLVV LFALVRANPP ANVTWIDQDG PVTVNTSDFL
   181  VLDAQNYPWL TNHTVQLQLR SLAHNLSVVA TNDVGVTSAS LPAPGLLATR VEVPLLGIVV
   241  AAGLALGTLV GFSTLVACLV CRKEKKTKGP SRHPSLISSD SNNLKLNNVR LPRENMSLPS
   301  NLQLNDLTPD SRAVKPADRQ MAQNNSRPEL LDPEPGGLLT SQGFIRLPVL GYIYRVSSVS
   361  SDEIWL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 49 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 49 nTPM
duodenum: 43 nTPM
cerebral cortex: 42 nTPM
basal ganglia: 40 nTPM
small intestine: 33 nTPM
hypothalamus: 29 nTPM

Single-cell type

enterocytes: 143 nCPM
oocytes: 69 nCPM
breast myoepithelial cells: 47 nCPM
cytotrophoblasts: 46 nCPM
breast hormone-responsive cells: 41 nCPM
paneth cells: 39 nCPM

Immune cell

naive CD4 T-cell: 10 nTPM
NK-cell: 7.4 nTPM
memory CD4 T-cell: 7.3 nTPM
MAIT T-cell: 7.1 nTPM
T-reg: 6 nTPM
naive CD8 T-cell: 5.4 nTPM

Brain region

cerebral cortex: 63 nTPM
hypothalamus: 51 nTPM
basal ganglia: 48 nTPM
white matter: 45 nTPM
cerebellum: 45 nTPM
amygdala: 43 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.56
gnomAD pLI: 0
gnomAD missense Z: 0.31
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMEM25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM25 as an antibody target. Whether an autoantibody or antibody against TMEM25 could matter depends on whether native TMEM25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM25 is annotated at the cell surface, where native TMEM25 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TMEM25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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