Seroatlas · Human Serome Atlas

TMEM225

Transmembrane protein 225

Also known as: PMP22CD, PPP1R154, SPATA47, TM225_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6GV28
Gene: TMEM225
Ensembl: ENSG00000204300
Chromosome: 11
Canonical length: 225 aa
Protein class: Predicted membrane proteins

OverviewNCBI Gene

Predicted to enable protein phosphatase 1 binding activity and protein phosphatase inhibitor activity. Predicted to be involved in negative regulation of phosphatase activity. Predicted to be located in inner acrosomal membrane and outer acrosomal membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

225 residues, UniProt reviewed canonical sequence.

>Q6GV28|TMEM225
     1  MVHVSNRSIQ GMNILFSSWA VVLMVMGITL DKWVELISED ERAKMNHSPW MMCCPALWPE
    61  DDLKVVRIMM TSSLGLSFLL NLILGMKFTY LIPQNKYIQL FTTILSFFSG ISLLWALILY
   121  HNKLKQGQSM HFSSYRITWI MYTAYLNVFF LSVCGVLSLL ECKLSTSSCT CLNIHKSDNE
   181  CKESENSIED ISLPECTAMP RSIVRAHTVN SLNKKVQTRH VTWAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM225 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 4
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

testis: 21 nTPM
adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM
basal ganglia: 0 nTPM

Single-cell type

late primary spermatocytes: 234 nCPM
early spermatids: 101 nCPM
late spermatids: 29 nCPM
early primary spermatocytes: 2.8 nCPM
granulosa cells: 1.6 nCPM
vascular endothelial cells: 1.5 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebral cortex: 0.4 nTPM
hypothalamus: 0.4 nTPM
amygdala: 0.3 nTPM
basal ganglia: 0.3 nTPM
hippocampal formation: 0.1 nTPM
medulla oblongata: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.42
gnomAD pLI: 0
gnomAD missense Z: 0.34
DepMap mean gene effect: 0.21
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 1% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

acrosomal membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMEM225 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

PPP1CC

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM225 as an antibody target. Whether an autoantibody or antibody against TMEM225 could matter depends on whether native TMEM225 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM225 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM225 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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