TKTL1

Protein identityUniProt · HPA

UniProt accession

P51854

Gene

TKTL1

Ensembl

ENSG00000007350

Chromosome

X

Canonical length

596 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

596 residues, UniProt reviewed canonical sequence.

>P51854|TKTL1
     1  MADAEARAEF PEEARPDRGT LQVLQDMASR LRIHSIRATC STSSGHPTSC SSSSEIMSVL
    61  FFYIMRYKQS DPENPDNDRF VLAKRLSFVD VATGWLGQGL GVACGMAYTG KYFDRASYRV
   121  FCLMSDGESS EGSVWEAMAF ASYYSLDNLV AIFDVNRLGH SGALPAEHCI NIYQRRCEAF
   181  GWNTYVVDGR DVEALCQVFW QASQVKHKPT AVVAKTFKGR GTPSIEDAES WHAKPMPRER
   241  ADAIIKLIES QIQTSRNLDP QPPIEDSPEV NITDVRMTSP PDYRVGDKIA TRKACGLALA
   301  KLGYANNRVV VLDGDTRYST FSEIFNKEYP ERFIECFMAE QNMVSVALGC ASRGRTIAFA
   361  STFAAFLTRA FDHIRIGGLA ESNINIIGSH CGVSVGDDGA SQMALEDIAM FRTIPKCTIF
   421  YPTDAVSTEH AVALAANAKG MCFIRTTRPE TMVIYTPQER FEIGQAKVLR HCVSDKVTVI
   481  GAGITVYEAL AAADELSKQD IFIRVIDLFT IKPLDVATIV SSAKATEGRI ITVEDHYPQG
   541  GIGEAVCAAV SMDPDIQVHS LAVSGVPQSG KSEELLDMYG ISARHIIVAV KCMLLN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TKTL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.21

Highest tissue expression

119 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.14

gnomAD pLI

1

gnomAD missense Z

-0.85

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• glucose catabolic process
• thiamine metabolic process

Molecular functions

• metal ion binding
• thiamine pyrophosphate binding
• transketolase activity

Cellular components

• cytosol

Protein domainsUniProt · Pfam · InterPro

• Transketolase, N-terminal
• Transketolase-like, pyrimidine-binding domain
• Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II
• Transketolase binding site
• Thiamin diphosphate-binding fold
• Transketolase, C-terminal domain
• Transketolase-like
• Transketolase, thiamine diphosphate binding domain
• Transketolase, pyrimidine binding domain
• Transketolase, C-terminal domain

KeywordsUniProt

• Calcium
• Cytoplasm
• Developmental protein
• Magnesium
• Metal-binding
• Thiamine pyrophosphate
• Transferase