Seroatlas · Human Serome Atlas

TKT

Transketolase

Also known as: TKT_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P29401
Gene
TKT
Ensembl
ENSG00000163931
Chromosome
3
Canonical length
623 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Canonical amino-acid sequenceUniProt

623 residues, UniProt reviewed canonical sequence.

>P29401|TKT
     1  MESYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAV LFFHTMRYKS
    61  QDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLAEAELLNL RKISSDLDGH PVPKQAFTDV
   121  ATGSLGQGLG AACGMAYTGK YFDKASYRVY CLLGDGELSE GSVWEAMAFA SIYKLDNLVA
   181  ILDINRLGQS DPAPLQHQMD IYQKRCEAFG WHAIIVDGHS VEELCKAFGQ AKHQPTAIIA
   241  KTFKGRGITG VEDKESWHGK PLPKNMAEQI IQEIYSQIQS KKKILATPPQ EDAPSVDIAN
   301  IRMPSLPSYK VGDKIATRKA YGQALAKLGH ASDRIIALDG DTKNSTFSEI FKKEHPDRFI
   361  ECYIAEQNMV SIAVGCATRN RTVPFCSTFA AFFTRAFDQI RMAAISESNI NLCGSHCGVS
   421  IGEDGPSQMA LEDLAMFRSV PTSTVFYPSD GVATEKAVEL AANTKGICFI RTSRPENAII
   481  YNNNEDFQVG QAKVVLKSKD DQVTVIGAGV TLHEALAAAE LLKKEKINIR VLDPFTIKPL
   541  DRKLILDSAR ATKGRILTVE DHYYEGGIGE AVSSAVVGEP GITVTHLAVN RVPRSGKPAE
   601  LLKMFGIDRD AIAQAVRGLI TKA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TKT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.19
Highest tissue expression
630 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 630 nTPM
  • esophagus: 160 nTPM
  • pancreas: 146 nTPM
  • adipose tissue: 143 nTPM
  • urinary bladder: 122 nTPM
  • thyroid gland: 110 nTPM

Single-cell type

  • esophageal basal cells: 1,041 nCPM
  • ocular epithelial cells: 1,019 nCPM
  • alveolar cells type 1: 935 nCPM
  • neutrophil progenitors: 804 nCPM
  • neutrophils: 791 nCPM
  • kupffer cells: 615 nCPM

Immune cell

  • classical monocyte: 447 nTPM
  • neutrophil: 376 nTPM
  • total PBMC: 312 nTPM
  • eosinophil: 275 nTPM
  • non-classical monocyte: 243 nTPM
  • myeloid DC: 204 nTPM

Brain region

  • hypothalamus: 129 nTPM
  • white matter: 126 nTPM
  • pons: 124 nTPM
  • cerebellum: 121 nTPM
  • midbrain: 116 nTPM
  • medulla oblongata: 115 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TKT.

Disease | AllUniProt

Conditions TKT is implicated in, by any mechanism.

  • Short stature, developmental delay, and congenital heart defects (SDDHD) MIM:617044

Disease | GeneticClinVar

5 pathogenic / likely-pathogenic of 159 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Transketolase deficiency

Disease | ImmuneIEDB

Conditions an epitope on TKT was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.56
gnomAD pLI
0.01
gnomAD missense Z
1.66
DepMap mean gene effect
-0.36
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TKT as an antibody target. Whether an autoantibody or antibody against TKT could matter depends on whether native TKT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TKT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TKT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TKT. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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