TIMM44

Protein identityUniProt · HPA

UniProt accession

O43615

Gene

TIMM44

Ensembl

ENSG00000104980

Chromosome

19

Canonical length

452 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

This gene encodes a peripheral membrane protein associated with the mitochondrial inner membrane translocase, which functions in the import of proteins across the mitochondrial inner membrane and into the mitochondrial matrix. The encoded protein mediates binding of mitochondrial heat shock protein 70 to the translocase of inner mitochondrial membrane 23 (TIM23) complex. Expression of this gene is upregulated in kidney in a mouse model of diabetes. A mutation in this gene is associated with familial oncocytic thyroid carcinoma. [provided by RefSeq, Jul 2016]

Canonical amino-acid sequenceUniProt

452 residues, UniProt reviewed canonical sequence.

>O43615|TIMM44
     1  MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS YSSGNRKGFL
    61  SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA RRKYKTIESE TVRTSEVLRK
   121  KLGELTGTVK ESLHEVSKSD LGRKIKEGVE EAAKTAKQSA ESVSKGGEKL GRTAAFRALS
   181  QGVESVKKEI DDSVLGQTGP YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK
   241  WYQQWKDFKE NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL
   301  TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ LAHPIQQAKA
   361  LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV VRNPKGEVVE GDPDKVLRML
   421  YVWALCRDQD ELNPYAAWRL LDISASSTEQ IL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TIMM44 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.44

Highest tissue expression

70 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.6

gnomAD pLI

0

gnomAD missense Z

0.23

DepMap mean gene effect

-0.8

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intracellular protein transport
• protein import into mitochondrial matrix
• protein targeting to mitochondrion

Molecular functions

• ATP binding
• protein-folding chaperone binding

Cellular components

• fibrillar center
• mitochondrial inner membrane
• mitochondrial matrix
• mitochondrion
• TIM23 mitochondrial import inner membrane translocase complex

Protein domainsUniProt · Pfam · InterPro

• Tim44-like domain
• NTF2-like domain superfamily
• Tim44-like domain
• Mitochondrial import inner membrane translocase subunit Tim44
• Tim44-like

KeywordsUniProt

• ATP-binding
• Membrane
• Mitochondrion
• Mitochondrion inner membrane
• Nucleotide-binding
• Phosphoprotein
• Protein transport
• Transit peptide
• Translocation
• Transport

InteractionsUniProt · HPA

Protein binding partners of TIMM44 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SLC25A5
• SLC25A4