Seroatlas · Human Serome Atlas

THUMPD3

tRNA (guanine(6)-N(2))-methyltransferase THUMP3

Also known as: DKFZP434F091, THUM3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BV44
Gene
THUMPD3
Ensembl
ENSG00000134077
Chromosome
3
Canonical length
507 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoli,Cytosol

OverviewNCBI Gene

Enables tRNA (guanine(6)-N2)-methyltransferase activity and tRNA (guanine(7)-N2)-methyltransferase activity. Involved in tRNA methylation. Located in cytosol and nucleolus. Part of tRNA methyltransferase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

507 residues, UniProt reviewed canonical sequence.

>Q9BV44|THUMPD3
     1  MCDIEEATNQ LLDVNLHENQ KSVQVTESDL GSESELLVTI GATVPTGFEQ TAADEVREKL
    61  GSSCKISRDR GKIYFVISVE SLAQVHCLRS VDNLFVVVQE FQDYQFKQTK EEVLKDFEDL
   121  AGKLPWSNPL KVWKINASFK KKKAKRKKIN QNSSKEKINN GQEVKIDQRN VKKEFTSHAL
   181  DSHILDYYEN PAIKEDVSTL IGDDLASCKD ETDESSKEET EPQVLKFRVT CNRAGEKHCF
   241  TSNEAARDFG GAVQDYFKWK ADMTNFDVEV LLNIHDNEVI VGIALTEESL HRRNITHFGP
   301  TTLRSTLAYG MLRLCDPLPY DIIVDPMCGT GAIPIEGATE WSDCFHIAGD NNPLAVNRAA
   361  NNIASLLTKS QIKEGKPSWG LPIDAVQWDI CNLPLRTGSV DIIVTDLPFG KRMGSKKRNW
   421  NLYPACLREM SRVCTPTTGR AVLLTQDTKC FTKALSGMRH VWRKVDTVWV NVGGLRAAVY
   481  VLIRTPQAFV HPSEQDGERG TLWQCKE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against THUMPD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
55 nTPM

Expression across tissuesHPA

Tissue

  • testis: 55 nTPM
  • bone marrow: 31 nTPM
  • skeletal muscle: 25 nTPM
  • tongue: 25 nTPM
  • parathyroid gland: 24 nTPM
  • heart muscle: 23 nTPM

Single-cell type

  • late primary spermatocytes: 392 nCPM
  • early spermatids: 261 nCPM
  • late spermatids: 177 nCPM
  • cardiomyocytes: 107 nCPM
  • epicardial cells: 100 nCPM
  • early primary spermatocytes: 89 nCPM

Immune cell

  • non-classical monocyte: 29 nTPM
  • naive CD4 T-cell: 28 nTPM
  • MAIT T-cell: 26 nTPM
  • intermediate monocyte: 24 nTPM
  • memory CD8 T-cell: 22 nTPM
  • naive CD8 T-cell: 22 nTPM

Brain region

  • white matter: 32 nTPM
  • choroid plexus: 31 nTPM
  • cerebellum: 28 nTPM
  • pons: 27 nTPM
  • hypothalamus: 26 nTPM
  • spinal cord: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.25
gnomAD pLI
0
gnomAD missense Z
-0.41
DepMap mean gene effect
0.13
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of THUMPD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads THUMPD3 as an antibody target. Whether an autoantibody or antibody against THUMPD3 could matter depends on whether native THUMPD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

THUMPD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label THUMPD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/THUMPD3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...