TFF3
Trefoil factor 3
Also known as: HITF, ITF, TFF3_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q07654
- Gene
- TFF3
- Ensembl
- ENSG00000160180
- Chromosome
- 21
- Canonical length
- 80 aa
- Protein class
- Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Predicted secreted proteins
- Subcellular location
- Nucleoplasm,Nucleoli
- Secretome location
- Secreted to blood
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
80 residues, UniProt reviewed canonical sequence.
>Q07654|TFF3
1 MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK ECNNRGCCFD
61 SRIPGVPWCF KPLQEAECTFLocalizationUniProt · AlphaFold · HPA
Whether an antibody against TFF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Secreted
- Secreted
- Yes
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.47
- Highest tissue expression
- 559 nTPM
Expression across tissuesHPA
Tissue
- thyroid gland: 559 nTPM
- salivary gland: 534 nTPM
- colon: 510 nTPM
- cervix: 434 nTPM
- rectum: 433 nTPM
- small intestine: 408 nTPM
Single-cell type
- goblet cells: 56,754 nCPM
- respiratory secretory cells: 2,948 nCPM
- conjunctival goblet cells: 2,501 nCPM
- lymphatic endothelial cells: 2,204 nCPM
- fallopian tube ciliated cells: 2,070 nCPM
- paneth cells: 1,914 nCPM
Immune cell
- eosinophil: 1.6 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- midbrain: 4.6 nTPM
- cerebellum: 0.7 nTPM
- thalamus: 0.6 nTPM
- medulla oblongata: 0.4 nTPM
- spinal cord: 0.4 nTPM
- basal ganglia: 0.3 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.59
- gnomAD pLI
- 0.02
- gnomAD missense Z
- 0.51
- DepMap mean gene effect
- 0
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads TFF3 as an antibody target. Whether an autoantibody or antibody against TFF3 could matter depends on whether native TFF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
TFF3 is annotated as secreted, so native TFF3 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.
Annotation status
The present source text does not explicitly label TFF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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