Seroatlas · Human Serome Atlas

SPHKAP

A-kinase anchor protein SPHKAP

Also known as: SKIP, SPKAP_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q2M3C7
Gene: SPHKAP
Ensembl: ENSG00000153820
Chromosome: 2
Canonical length: 1700 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Enables protein kinase A binding activity. Predicted to be located in Z disc. Predicted to be active in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1700 residues, UniProt reviewed canonical sequence.

>Q2M3C7|SPHKAP
     1  MDGNSLLSVP SNLESSRMYD VLEPQQGRGC GSSGSGPGNS ITACKKVLRS NSLLESTDYW
    61  LQNQRMPCQI GFVEDKSENC ASVCFVNLDV NKDECSTEHL QQKLVNVSPD LPKLISSMNV
   121  QQPKENEIVV LSGLASGNLQ ADFEVSQCPW LPDICLVQCA RGNRPNSTNC IIFEINKFLI
   181  GLELVQERQL HLETNILKLE DDTNCSLSSI EEDFLTASEH LEEESEVDES RNDYENINVS
   241  ANVLESKQLK GATQVEWNCN KEKWLYALED KYINKYPTPL IKTERSPENL TKNTALQSLD
   301  PSAKPSQWKR EAVGNGRQAT HYYHSEAFKG QMEKSQALYI PKDAYFSMMD KDVPSACAVA
   361  EQRSNLNPGD HEDTRNALPP RQDGEVTTGK YATNLAESVL QDAFIRLSQS QSTLPQESAV
   421  SVSVGSSLLP SCYSTKDTVV SRSWNELPKI VVVQSPDGSD AAPQPGISSW PEMEVSVETS
   481  SILSGENSSR QPQSALEVAL ACAATVIGTI SSPQATERLK MEQVVSNFPP GSSGALQTQA
   541  PQGLKEPSIN EYSFPSALCG MTQVASAVAV CGLGEREEVT CSVAPSGSLP PAAEASEAMP
   601  PLCGLASMEL GKEAIAKGLL KEAALVLTRP NTYSSIGDFL DSMNRRIMET ASKSQTLCSE
   661  NVVRNELAHT LSNVILRHSI DEVHHKNMII DPNDNRHSSE ILDTLMESTN QLLLDVICFT
   721  FKKMSHIVRL GECPAVLSKE TIRRRETEPS CQPSDPGASQ AWTKATESSS SSPLSNSHNT
   781  SLVINNLVDG MYSKQDKGGV RPGLFKNPTL QSQLSRSHRV PDSSTATTSS KEIYLKGIAG
   841  EDTKSPHHSE NECRASSEGQ RSPTVSQSRS GSQEAEESIH PNTQEKYNCA TSRINEVQVN
   901  LSLLGDDLLL PAQSTLQTKH PDIYCITDFA EELADTVVSM ATEIAAICLD NSSGKQPWFC
   961  AWKRGSEFLM TPNVPCRSLK RKKESQGSGT AVRKHKPPRL SEIKRKTDEH PELKEKLMNR
  1021  VVDESMNLED VPDSVNLFAN EVAAKIMNLT EFSMVDGMWQ AQGYPRNRLL SGDRWSRLKA
  1081  SSCESIPEED SEARAYVNSL GLMSTLSQPV SRASSVSKQS SCESITDEFS RFMVNQMENE
  1141  GRGFELLLDY YAGKNASSIL NSAMQQACRK SDHLSVRPSC PSKQSSTESI TEEFYRYMLR
  1201  DIERDSRESA SSRRSSQDWT AGLLSPSLRS PVCHRQSSMP DSRSPCSRLT VNVPIKANSL
  1261  DGFAQNCPQD FLSVQPVSSA SSSGLCKSDS CLYRRGGTDH ITNMLIHETW ASSIEALMRK
  1321  NKIIVDDAEE ADTEPVSGGS PSQAEKCANR LAASRMCSGP TLLVQESLDC PRKDSVTECK
  1381  QPPVSSLSKT ASLTNHSPLD SKKETSSCQD PVPINHKRRS LCSREVPLIQ IETDQREACA
  1441  GEPEPFLSKS SLLEEAEGHS NDKNIPDVVR GGDTAVSACQ IHSDSLDTRD VPEAEASTEA
  1501  RAPDEAPNPP SSSEESTGSW TQLANEEDNP DDTSSFLQLS ERSMSNGNSS ATSSLGIMDL
  1561  DIYQESMPSS PMINELVEEK KILKGQSEST EAPASGPPTG TASPQRSLLV INFDLEPECP
  1621  DAELRATLQW IAASELGIPT IYFKKSQENR IEKFLDVVQL VHRKSWKVGD IFHAVVQYCK
  1681  MHEEQKDGRL SLFDWLLELG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPHKAP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.58
Highest tissue expression: 34 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 34 nTPM
heart muscle: 27 nTPM
hippocampal formation: 14 nTPM
cerebral cortex: 11 nTPM
hypothalamus: 4.1 nTPM
amygdala: 3.8 nTPM

Single-cell type

cardiomyocytes: 466 nCPM
brain excitatory neurons: 425 nCPM
brain inhibitory neurons: 279 nCPM
other brain neurons: 278 nCPM
retinal amacrine cells: 219 nCPM
granulosa cells: 131 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 173 nTPM
cerebral cortex: 118 nTPM
hippocampal formation: 96 nTPM
midbrain: 36 nTPM
thalamus: 32 nTPM
amygdala: 31 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.32
gnomAD pLI: 0.94
gnomAD missense Z: -0.76
DepMap mean gene effect: 0.11
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

protein kinase A binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPHKAP in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

SPHK1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPHKAP as an antibody target. Whether an autoantibody or antibody against SPHKAP could matter depends on whether native SPHKAP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPHKAP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPHKAP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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