Seroatlas · Human Serome Atlas

SPATA20

Spermatogenesis-associated protein 20

Also known as: FLJ21347, SPT20_HUMAN, SSP411, Tisp78

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8TB22
Gene: SPATA20
Ensembl: ENSG00000006282
Chromosome: 17
Canonical length: 786 aa
Protein class: Predicted intracellular proteins, Predicted secreted proteins
Subcellular location: Principal piece
Secretome location: Secreted in male reproductive system

OverviewNCBI Gene

Predicted to be involved in carbohydrate metabolic process; cell differentiation; and spermatogenesis. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

786 residues, UniProt reviewed canonical sequence.

>Q8TB22|SPATA20
     1  MLGARAWLGR VLLLPRAGAG LAASRRGSSS RDKDRSATVS SSVPMPAGGK GSHPSSTPQR
    61  VPNRLIHEKS PYLLQHAYNP VDWYPWGQEA FDKARKENKP IFLSVGYSTC HWCHMMEEES
   121  FQNEEIGRLL SEDFVSVKVD REERPDVDKV YMTFVQATSS GGGWPMNVWL TPNLQPFVGG
   181  TYFPPEDGLT RVGFRTVLLR IREQWKQNKN TLLENSQRVT TALLARSEIS VGDRQLPPSA
   241  ATVNNRCFQQ LDEGYDEEYG GFAEAPKFPT PVILSFLFSY WLSHRLTQDG SRAQQMALHT
   301  LKMMANGGIR DHVGQGFHRY STDRQWHVPH FEKMLYDQAQ LAVAYSQAFQ LSGDEFYSDV
   361  AKGILQYVAR SLSHRSGGFY SAEDADSPPE RGQRPKEGAY YVWTVKEVQQ LLPEPVLGAT
   421  EPLTSGQLLM KHYGLTEAGN ISPSQDPKGE LQGQNVLTVR YSLELTAARF GLDVEAVRTL
   481  LNSGLEKLFQ ARKHRPKPHL DSKMLAAWNG LMVSGYAVTG AVLGQDRLIN YATNGAKFLK
   541  RHMFDVASGR LMRTCYTGPG GTVEHSNPPC WGFLEDYAFV VRGLLDLYEA SQESAWLEWA
   601  LRLQDTQDKL FWDSQGGGYF CSEAELGAGL PLRLKDDQDG AEPSANSVSA HNLLRLHGFT
   661  GHKDWMDKCV CLLTAFSERM RRVPVALPEM VRALSAQQQT LKQIVICGDR QAKDTKALVQ
   721  CVHSVYIPNK VLILADGDPS SFLSRQLPFL STLRRLEDQA TAYVCENQAC SVPITDPCEL
   781  RKLLHP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPATA20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.23
Highest tissue expression: 113 nTPM

Expression across tissuesHPA

Tissue

testis: 113 nTPM
choroid plexus: 50 nTPM
adrenal gland: 46 nTPM
cerebral cortex: 34 nTPM
heart muscle: 32 nTPM
cerebellum: 30 nTPM

Single-cell type

late spermatids: 2,405 nCPM
early spermatids: 663 nCPM
late primary spermatocytes: 190 nCPM
foveolar cells: 70 nCPM
tuft cells: 69 nCPM
parietal cells: 60 nCPM

Immune cell

myeloid DC: 8.3 nTPM
NK-cell: 7.4 nTPM
classical monocyte: 5 nTPM
intermediate monocyte: 4.5 nTPM
total PBMC: 3.7 nTPM
non-classical monocyte: 3.4 nTPM

Brain region

thalamus: 42 nTPM
choroid plexus: 35 nTPM
cerebral cortex: 32 nTPM
midbrain: 32 nTPM
hypothalamus: 30 nTPM
pons: 30 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.7
gnomAD pLI: 0
gnomAD missense Z: 0.12
DepMap mean gene effect: -0.09
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

Six-hairpin glycosidase superfamily
Six-hairpin glycosidase-like superfamily
Thioredoxin-like superfamily
Spermatogenesis-associated protein 20-like, TRX domain
Spermatogenesis-associated protein 20
Protein of unknown function, DUF255

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPATA20 as an antibody target. Whether an autoantibody or antibody against SPATA20 could matter depends on whether native SPATA20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPATA20 is annotated as secreted, so native SPATA20 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label SPATA20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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