Seroatlas · Human Serome Atlas

SMYD3

Histone-lysine N-methyltransferase SMYD3

Also known as: KMT3E, SMYD3_HUMAN, ZMYND1, ZNFN3A1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H7B4
Gene
SMYD3
Ensembl
ENSG00000185420
Chromosome
1
Canonical length
428 aa
Protein class
Cancer-related genes, Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Canonical amino-acid sequenceUniProt

428 residues, UniProt reviewed canonical sequence.

>Q9H7B4|SMYD3
     1  MEPLKVEKFA TAKRGNGLRA VTPLRPGELL FRSDPLAYTV CKGSRGVVCD RCLLGKEKLM
    61  RCSQCRVAKY CSAKCQKKAW PDHKRECKCL KSCKPRYPPD SVRLLGRVVF KLMDGAPSES
   121  EKLYSFYDLE SNINKLTEDK KEGLRQLVMT FQHFMREEIQ DASQLPPAFD LFEAFAKVIC
   181  NSFTICNAEM QEVGVGLYPS ISLLNHSCDP NCSIVFNGPH LLLRAVRDIE VGEELTICYL
   241  DMLMTSEERR KQLRDQYCFE CDCFRCQTQD KDADMLTGDE QVWKEVQESL KKIEELKAHW
   301  KWEQVLAMCQ AIISSNSERL PDINIYQLKV LDCAMDACIN LGLLEEALFY GTRTMEPYRI
   361  FFPGSHPVRG VQVMKVGKLQ LHQGMFPQAM KNLRLAFDIM RVTHGREHSL IEDLILLLEE
   421  CDANIRAS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SMYD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
21 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 21 nTPM
  • cerebellum: 19 nTPM
  • cerebral cortex: 15 nTPM
  • thyroid gland: 15 nTPM
  • basal ganglia: 15 nTPM
  • hypothalamus: 14 nTPM

Single-cell type

  • mast cells: 1,384 nCPM
  • thyrotrophs: 1,211 nCPM
  • cone photoreceptor cells: 1,161 nCPM
  • choroid plexus epithelial cells: 1,024 nCPM
  • brain excitatory neurons: 1,022 nCPM
  • brain inhibitory neurons: 957 nCPM

Immune cell

  • NK-cell: 28 nTPM
  • memory CD8 T-cell: 16 nTPM
  • T-reg: 16 nTPM
  • naive CD8 T-cell: 16 nTPM
  • naive B-cell: 16 nTPM
  • naive CD4 T-cell: 15 nTPM

Brain region

  • cerebellum: 44 nTPM
  • hypothalamus: 34 nTPM
  • midbrain: 32 nTPM
  • hippocampal formation: 32 nTPM
  • pons: 31 nTPM
  • basal ganglia: 30 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.9
gnomAD pLI
0
gnomAD missense Z
0.24
DepMap mean gene effect
0.07
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SMYD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SMYD3 as an antibody target. Whether an autoantibody or antibody against SMYD3 could matter depends on whether native SMYD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SMYD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SMYD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SMYD3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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