SEPHS2

Protein identityUniProt · HPA

UniProt accession

Q99611

Gene

SEPHS2

Ensembl

ENSG00000179918

Chromosome

16

Canonical length

448 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. A pseudogene for this locus has been identified on chromosome 5. [provided by RefSeq, May 2017]

Canonical amino-acid sequenceUniProt

448 residues, UniProt reviewed canonical sequence.

>Q99611|SEPHS2
     1  MAEASATGAC GEAMAAAEGS SGPAGLTLGR SFSNYRPFEP QALGLSPSWR LTGFSGMKGU
    61  GCKVPQEALL KLLAGLTRPD VRPPLGRGLV GGQEEASQEA GLPAGAGPSP TFPALGIGMD
   121  SCVIPLRHGG LSLVQTTDFF YPLVEDPYMM GRIACANVLS DLYAMGITEC DNMLMLLSVS
   181  QSMSEEEREK VTPLMVKGFR DAAEEGGTAV TGGQTVVNPW IIIGGVATVV CQPNEFIMPD
   241  SAVVGDVLVL TKPLGTQVAV NAHQWLDNPE RWNKVKMVVS REEVELAYQE AMFNMATLNR
   301  TAAGLMHTFN AHAATDITGF GILGHSQNLA KQQRNEVSFV IHNLPIIAKM AAVSKASGRF
   361  GLLQGTSAET SGGLLICLPR EQAARFCSEI KSSKYGEGHQ AWIVGIVEKG NRTARIIDKP
   421  RVIEVLPRGA TAAVLAPDSS NASSEPSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SEPHS2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0

Highest tissue expression

377 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1

gnomAD pLI

0

gnomAD missense Z

1.76

DepMap mean gene effect

-0.7

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• selenium compound metabolic process
• selenocysteine biosynthetic process
• selenocysteine metabolic process

Molecular functions

• ATP binding
• metal ion binding
• selenide, water dikinase activity

Cellular components

• cytoplasm
• cytosol

Protein domainsUniProt · Pfam · InterPro

• Selenophosphate synthetase
• PurM-like, C-terminal domain
• PurM-like, N-terminal domain
• PurM-like, C-terminal domain superfamily
• PurM-like, N-terminal domain superfamily
• AIR synthase related protein, N-terminal domain
• AIR synthase related protein, C-terminal domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Kinase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Selenium
• Selenocysteine
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of SEPHS2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SEPHS1