Seroatlas · Human Serome Atlas

SCML4

Sex comb on midleg-like protein 4

Also known as: dJ47M23.1, SCML4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N228
Gene: SCML4
Ensembl: ENSG00000146285
Chromosome: 6
Canonical length: 414 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable chromatin binding activity and histone binding activity. Predicted to be involved in negative regulation of DNA-templated transcription. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

414 residues, UniProt reviewed canonical sequence.

>Q8N228|SCML4
     1  MQSQRIPGRK RGRPSLHSTP MKMAVHNLYS ASAGSLPAVK IPKKRGRKPG YKIKSRVLMT
    61  PLALSPPRST PEPDLSSIPQ DAATVPSLAA PQALTVCLYI NKQANAGPYL ERKKVQQLPE
   121  HFGPERPSAV LQQAVQACID CAHQQKLVFS LVKQGYGGEM VSVSASFDGK QHLRSLPVVN
   181  SIGYVLRFLA KLCRSLLCDD LFSHQPFPRG CSASEKVQEK EEGRMESVKT VTTEEYLVNP
   241  VGMNRYSVDT SASTFNHRGS LHPSSSLYCK RQNSGDSHLG GGPAATAGGP RTSPMSSGGP
   301  SAPGLRPPAS SPKRNTTSLE GNRCASSPSQ DAQDARRPRS RNPSAWTVED VVWFVKDADP
   361  QALGPHVELF RKHEIDGNAL LLLKSDMVMK YLGLKLGPAL KLCYHIDKLK QAKF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SCML4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

lymph node: 11 nTPM
duodenum: 9.5 nTPM
tonsil: 9.2 nTPM
thymus: 8.6 nTPM
small intestine: 7.4 nTPM
appendix: 6.8 nTPM

Single-cell type

t-cells: 385 nCPM
innate lymphoid cells: 331 nCPM
nk-cells: 318 nCPM
late spermatids: 280 nCPM
thymocytes: 257 nCPM
early spermatids: 172 nCPM

Immune cell

memory CD4 T-cell: 75 nTPM
naive CD4 T-cell: 69 nTPM
memory CD8 T-cell: 66 nTPM
naive CD8 T-cell: 60 nTPM
NK-cell: 54 nTPM
gdT-cell: 48 nTPM

Brain region

hypothalamus: 7.9 nTPM
cerebellum: 7.6 nTPM
cerebral cortex: 7.2 nTPM
basal ganglia: 6.8 nTPM
medulla oblongata: 6.4 nTPM
amygdala: 6.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.97
gnomAD pLI: 0
gnomAD missense Z: 0.68
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

negative regulation of DNA-templated transcription

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SCML4 as an antibody target. Whether an autoantibody or antibody against SCML4 could matter depends on whether native SCML4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SCML4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SCML4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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