Seroatlas · Human Serome Atlas

SARAF

Store-operated calcium entry-associated regulatory factor

Also known as: MGC8721, SARAF_HUMAN, TMEM66

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96BY9
Gene: SARAF
Ensembl: ENSG00000133872
Chromosome: 8
Canonical length: 339 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Endoplasmic reticulum,Rods & Rings

OverviewNCBI Gene

Involved in regulation of store-operated calcium entry. Located in endoplasmic reticulum membrane and endoplasmic reticulum-plasma membrane contact site. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

339 residues, UniProt reviewed canonical sequence.

>Q96BY9|SARAF
     1  MAAACGPGAA GYCLLLGLHL FLLTAGPALG WNDPDRMLLR DVKALTLHYD RYTTSRRLDP
    61  IPQLKCVGGT AGCDSYTPKV IQCQNKGWDG YDVQWECKTD LDIAYKFGKT VVSCEGYESS
   121  EDQYVLRGSC GLEYNLDYTE LGLQKLKESG KQHGFASFSD YYYKWSSADS CNMSGLITIV
   181  VLLGIAFVVY KLFLSDGQYS PPPYSEYPPF SHRYQRFTNS AGPPPPGFKS EFTGPQNTGH
   241  GATSGFGSAF TGQQGYENSG PGFWTGLGTG GILGYLFGSN RAATPFSDSW YYPSYPPSYP
   301  GTWNRAYSPL HGGSGSYSVC SNSDTKTRTA SGYGGTRRR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SARAF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.5
Highest tissue expression: 386 nTPM

Expression across tissuesHPA

Tissue

epididymis: 386 nTPM
cerebral cortex: 304 nTPM
hypothalamus: 299 nTPM
placenta: 291 nTPM
liver: 283 nTPM
choroid plexus: 282 nTPM

Single-cell type

late spermatids: 1,496 nCPM
t-cells: 1,185 nCPM
epididymal principal cells: 914 nCPM
early spermatids: 722 nCPM
mast cells: 661 nCPM
innate lymphoid cells: 656 nCPM

Immune cell

basophil: 969 nTPM
total PBMC: 925 nTPM
naive CD4 T-cell: 779 nTPM
naive CD8 T-cell: 546 nTPM
T-reg: 485 nTPM
neutrophil: 481 nTPM

Brain region

hypothalamus: 538 nTPM
midbrain: 438 nTPM
pons: 415 nTPM
cerebral cortex: 374 nTPM
hippocampal formation: 366 nTPM
basal ganglia: 359 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.83
gnomAD pLI: 0
DepMap mean gene effect: 0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

Store-operated calcium entry-associated regulatory factor
SOCE-associated regulatory factor of calcium homoeostasis

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SARAF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

STIM1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SARAF as an antibody target. Whether an autoantibody or antibody against SARAF could matter depends on whether native SARAF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SARAF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SARAF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...