Seroatlas · Human Serome Atlas

SAGE1

Sarcoma antigen 1

Also known as: CT14, SAGE, SAGE1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NXZ1
Gene: SAGE1
Ensembl: ENSG00000181433
Chromosome: X
Canonical length: 904 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene belongs to a class of genes that are activated in tumors. These genes are expressed in tumors of different histologic types but not in normal tissues, except for spermatogenic cells and, for some, placenta. The proteins encoded by these genes appear to be strictly tumor specific, and hence may be excellent sources of antigens for cancer immunotherapy. This gene is expressed in sarcomas. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

904 residues, UniProt reviewed canonical sequence.

>Q9NXZ1|SAGE1
     1  MQASPLQTSQ PTPPEELHAA AYVFTNDGQQ MRSDEVNLVA TGHQSKKKHS RKSKRHSSSK
    61  RRKSMSSWLD KQEDAAVTHS ICEERINNGQ PVADNVLSTA PPWPDATIAH NIREERMENG
   121  QSRTDKVLST APPQLVHMAA AGIPSMSTRD LHSTVTHNIR EERMENGQPQ PDNVLSTGPT
   181  GLINMAATPI PAMSARDLYA TVTHNVCEQK MENVQPAPDN VLLTLRPRRI NMTDTGISPM
   241  STRDPYATIT YNVPEEKMEK GQPQPDNILS TASTGLINVA GAGTPAISTN GLYSTVPHNV
   301  CEEKMENDQP QPNNVLSTVQ PVIIYLTATG IPGMNTRDQY ATITHNVCEE RVVNNQPLPS
   361  NALSTVLPGL AYLATADMPA MSTRDQHATI IHNLREEKKD NSQPTPDNVL SAVTPELINL
   421  AGAGIPPMST RDQYATVNHH VHEARMENGQ RKQDNVLSNV LSGLINMAGA SIPAMSSRDL
   481  YATITHSVRE EKMESGKPQT DKVISNDAPQ LGHMAAGGIP SMSTKDLYAT VTQNVHEERM
   541  ENNQPQPSYD LSTVLPGLTY LTVAGIPAMS TRDQYATVTH NVHEEKIKNG QAASDNVFST
   601  VPPAFINMAA TGVSSMSTRD QYAAVTHNIR EEKINNSQPA PGNILSTAPP WLRHMAAAGI
   661  SSTITRDLYV TATHSVHEEK MTNGQQAPDN SLSTVPPGCI NLSGAGISCR STRDLYATVI
   721  HDIQEEEMEN DQTPPDGFLS NSDSPELINM TGHCMPPNAL DSFSHDFTSL SKDELLYKPD
   781  SNEFAVGTKN YSVSAGDPPV TVMSLVETVP NTPQISPAMA KKINDDIKYQ LMKEVRRFGQ
   841  NYERIFILLE EVQGSMKVKR QFVEFTIKEA ARFKKVVLIQ QLEKALKEID SHCHLRKVKH
   901  MRKR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SAGE1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 4.5 nTPM

Expression across tissuesHPA

Tissue

testis: 4.5 nTPM
spinal cord: 0.3 nTPM
choroid plexus: 0.2 nTPM
amygdala: 0.1 nTPM
hippocampal formation: 0.1 nTPM
hypothalamus: 0.1 nTPM

Single-cell type

differentiating spermatogonia: 17 nCPM
undifferentiated spermatogonia: 8.1 nCPM
early primary spermatocytes: 4.2 nCPM
megakaryocyte-erythroid progenitors: 0.9 nCPM
mesothelial cells: 0.7 nCPM
early spermatids: 0.6 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

pons: 0.7 nTPM
white matter: 0.7 nTPM
medulla oblongata: 0.5 nTPM
midbrain: 0.5 nTPM
spinal cord: 0.4 nTPM
thalamus: 0.3 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SAGE1.

Disease | ImmuneIEDB

Conditions an epitope on SAGE1 was assayed in.

melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.32
gnomAD pLI: 0
gnomAD missense Z: -0.39
DepMap mean gene effect: 0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

snRNA 3'-end processing

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SAGE1 as an antibody target. Whether an autoantibody or antibody against SAGE1 could matter depends on whether native SAGE1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SAGE1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SAGE1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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