Seroatlas · Human Serome Atlas

RUFY2

RUN and FYVE domain-containing protein 2

Also known as: FLJ10063, KIAA1537, RABIP4R, RUFY2_HUMAN, ZFYVE13

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WXA3
Gene: RUFY2
Ensembl: ENSG00000204130
Chromosome: 10
Canonical length: 606 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables SH3 domain binding activity. Predicted to be involved in regulation of endocytosis. Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

606 residues, UniProt reviewed canonical sequence.

>Q8WXA3|RUFY2
     1  MATKDPTAVE RANLLNMAKL SIKGLIESAL SFGRTLDSDY PPLQQFFVVM EHCLKHGLKV
    61  RKSFLSYNKT IWGPLELVEK LYPEAEEIGA SVRDLPGLKT PLGRARAWLR LALMQKKMAD
   121  YLRCLIIQRD LLSEFYEYHA LMMEEEGAVI VGLLVGLNVI DANLCVKGED LDSQVGVIDF
   181  SMYLKNEEDI GNKERNVQIA AILDQKNYVE ELNRQLNSTV SSLHSRVDSL EKSNTKLIEE
   241  LAIAKNNIIK LQEENHQLRS ENKLILMKTQ QHLEVTKVDV ETELQTYKHS RQGLDEMYNE
   301  ARRQLRDESQ LRQDVENELA VQVSMKHEIE LAMKLLEKDI HEKQDTLIGL RQQLEEVKAI
   361  NIEMYQKLQG SEDGLKEKNE IIARLEEKTN KITAAMRQLE QRLQQAEKAQ MEAEDEDEKY
   421  LQECLSKSDS LQKQISQKEK QLVQLETDLK IEKEWRQTLQ EDLQKEKDAL SHLRNETQQI
   481  ISLKKEFLNL QDENQQLKKI YHEQEQALQE LGNKLSESKL KIEDIKEANK ALQGLVWLKD
   541  KEATHCKLCE KEFSLSKRKH HCRNCGEIFC NACSDNELPL PSSPKPVRVC DSCHALLIQR
   601  CSSNLP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RUFY2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 31 nTPM

Expression across tissuesHPA

Tissue

spinal cord: 31 nTPM
cerebellum: 30 nTPM
cerebral cortex: 18 nTPM
midbrain: 17 nTPM
hippocampal formation: 14 nTPM
hypothalamus: 14 nTPM

Single-cell type

oligodendrocytes: 194 nCPM
epicardial cells: 184 nCPM
retinal amacrine cells: 129 nCPM
fibro-adipogenic progenitors: 125 nCPM
brain excitatory neurons: 119 nCPM
adipocytes: 119 nCPM

Immune cell

NK-cell: 2.6 nTPM
naive CD8 T-cell: 1.4 nTPM
memory CD4 T-cell: 1.1 nTPM
memory CD8 T-cell: 1.1 nTPM
naive CD4 T-cell: 1.1 nTPM
eosinophil: 1 nTPM

Brain region

white matter: 40 nTPM
medulla oblongata: 30 nTPM
cerebellum: 28 nTPM
pons: 28 nTPM
basal ganglia: 27 nTPM
thalamus: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.5
gnomAD pLI: 0
gnomAD missense Z: 1.68
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of endocytosis

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

FYVE zinc finger
RUN domain
Zinc finger, FYVE/PHD-type
Zinc finger, RING/FYVE/PHD-type
Zinc finger, FYVE-related
RUN domain superfamily
RUN and FYVE domain-containing protein 1-3
FYVE zinc finger
RUN domain
RUN and FYVE domain-containing protein 2, RUN domain
RUN and FYVE domain-containing protein 2, FYVE domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RUFY2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RUFY2 as an antibody target. Whether an autoantibody or antibody against RUFY2 could matter depends on whether native RUFY2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RUFY2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RUFY2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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