Seroatlas · Human Serome Atlas

RSAD1

Radical S-adenosyl methionine domain-containing protein 1, mitochondrial

Also known as: FLJ11164, HemW, RSAD1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HA92
Gene: RSAD1
Ensembl: ENSG00000136444
Chromosome: 17
Canonical length: 442 aa
Protein class: Predicted intracellular proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

Enables heme binding activity. Predicted to be involved in porphyrin-containing compound biosynthetic process. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

442 residues, UniProt reviewed canonical sequence.

>Q9HA92|RSAD1
     1  MALPGARARG WAAAARAAQR RRRVENAGGS PSPEPAGRRA ALYVHWPYCE KRCSYCNFNK
    61  YIPRRLEEAA MQKCLVTEAQ TLLRLSGVQR VESVFFGGGT PSLASPHTVA AVLEAVAQAA
   121  HLPADLEVTL EANPTSAPGS RLAEFGAAGV NRLSIGLQSL DDTELRLLGR THSACDALRT
   181  LAEARRLFPG RVSVDLMLGL PAQQVGPWLG QLQELLHHCD DHLSLYQLSL ERGTALFAQV
   241  QRGALPAPDP ELAAEMYQRG RAVLREAGFH QYEVSNFARN GALSTHNWTY WQCGQYLGVG
   301  PGAHGRFMPQ GAGGHTREAR IQTLEPDNWM KEVMLFGHGT RKRVPLGRLE LLEEVLALGL
   361  RTDVGITHQH WQQFEPQLTL WDVFGANKEV QELLERGLLQ LDHRGLRCSW EGLAVLDSLL
   421  LTLLPQLQEA WQQRTPSPVP GG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RSAD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 65 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 65 nTPM
cerebellum: 57 nTPM
tongue: 53 nTPM
pancreas: 48 nTPM
liver: 44 nTPM
heart muscle: 42 nTPM

Single-cell type

tuft cells: 69 nCPM
proximal tubule cells: 56 nCPM
müller glia: 41 nCPM
adrenal cortex cells: 38 nCPM
differentiating spermatogonia: 36 nCPM
cytotrophoblasts: 30 nCPM

Immune cell

naive CD8 T-cell: 9.5 nTPM
naive CD4 T-cell: 9.4 nTPM
gdT-cell: 8.5 nTPM
MAIT T-cell: 8.5 nTPM
memory CD8 T-cell: 8.4 nTPM
memory CD4 T-cell: 7.3 nTPM

Brain region

basal ganglia: 26 nTPM
cerebellum: 26 nTPM
cerebral cortex: 25 nTPM
white matter: 24 nTPM
thalamus: 22 nTPM
pons: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.98
gnomAD pLI: 0
gnomAD missense Z: -0.37
DepMap mean gene effect: -0.38
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

porphyrin-containing compound biosynthetic process

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Elp3/MiaA/NifB-like, radical SAM core domain
Radical SAM
Aldolase-type TIM barrel
Radical SAM superfamily
Radical SAM superfamily
Heme chaperone HemW-like
HemN, C-terminal
Anaerobic coproporphyrinogen-III oxidase
HemN C-terminal domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RSAD1 as an antibody target. Whether an autoantibody or antibody against RSAD1 could matter depends on whether native RSAD1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RSAD1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RSAD1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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