RNF217

Protein identityUniProt · HPA

UniProt accession

Q8TC41

Gene

RNF217

Ensembl

ENSG00000146373

Chromosome

6

Canonical length

542 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

This protein encoded by this gene is a member of the RING1-IBR-RING24 (RBR) ubiquitin protein ligase family, and it belongs to a subfamily of these proteins that contain a transmembrane domain. This protein can interact with the HAX1 anti-apoptotic protein via its C-terminal RING finger motif, which suggests a role in apoptosis signaling. It is thought that deregulation of this gene can be a mechanism in leukemogenesis. Mutations in the region encoding the protein GXXXG motif, which appears to be necessary for protein self-association, have been found in human cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016]

Canonical amino-acid sequenceUniProt

542 residues, UniProt reviewed canonical sequence.

>Q8TC41|RNF217
     1  MGEEQSTVSG GGGPQESQTL ASGTAGHPEP PRPQGDSARA PPLRAASAEP SGGGCGSDWG
    61  CADTSAPEPA RSLGPPGWSK SRAPAQPAGL ALTGPLNPQT LPLQLELEEE EEEAGDRKEG
   121  GDEQQEAPPG EELEPRTRVG AADGLVLDVL GQRRPSLAKR QVFCSVYCVE SDLPEAPASE
   181  QLSPPASPPG APPVLNPPST RSSFPSPRLS LPTDSLSPDG GSIELEFYLA PEPFSMPSLL
   241  GAPPYSGLGG VGDPYVPLMV LMCRVCLEDK PIKPLPCCKK AVCEECLKVY LSAQVQLGQV
   301  EIKCPITECF EFLEETTVVY NLTHEDSIKY KYFLELGRID SSTKPCPQCK HFTTFKKKGH
   361  IPTPSRSESK YKIQCPTCQF VWCFKCHSPW HEGVNCKEYK KGDKLLRHWA SEIEHGQRNA
   421  QKCPKCKIHI QRTEGCDHMT CSQCNTNFCY RCGERYRQLR FFGDHTSNLS IFGCKYRYLP
   481  ERPHLRRLVR GSVCAGKLFI APLIMVLGLA LGAIAVVIGL FVFPIYCLCK KQRKRSRTGM
   541  HW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF217 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.54

Highest tissue expression

39 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1

gnomAD pLI

0

gnomAD missense Z

1.31

DepMap mean gene effect

0.03

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• protein ubiquitination
• ubiquitin-dependent protein catabolic process

Molecular functions

• ubiquitin conjugating enzyme binding
• ubiquitin protein ligase activity
• ubiquitin-protein transferase activity
• zinc ion binding

Cellular components

• cytoplasm
• cytosol
• membrane
• ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• IBR domain
• Zinc finger, RING/FYVE/PHD-type
• E3 ubiquitin ligase RBR family
• TRIAD supradomain
• IBR domain, a half RING-finger domain
• IBR domain
• E3 ubiquitin-protein ligase RNF217, variant RING finger, HC subclass
• E3 ubiquitin-protein ligase RNF217, BRcat domain
• E3 ubiquitin-protein ligase RNF217, Rcat domain

KeywordsUniProt

• Cytoplasm
• Membrane
• Metal-binding
• Repeat
• Transferase
• Transmembrane
• Transmembrane helix
• Ubl conjugation pathway
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of RNF217 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HAX1