Seroatlas · Human Serome Atlas

RNF186

E3 ubiquitin-protein ligase RNF186

Also known as: FLJ20225, RN186_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NXI6
Gene: RNF186
Ensembl: ENSG00000178828
Chromosome: 1
Canonical length: 227 aa
Protein class: Enzymes, Predicted membrane proteins

OverviewNCBI Gene

Enables ubiquitin protein ligase activity and ubiquitin protein ligase binding activity. Involved in several processes, including intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein ubiquitination. Acts upstream of with a positive effect on regulation of autophagosome assembly. Located in endoplasmic reticulum membrane. Is active in endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

227 residues, UniProt reviewed canonical sequence.

>Q9NXI6|RNF186
     1  MACTKTLQQS QPISAGATTT TTAVAPAGGH SGSTECDLEC LVCREPYSCP RLPKLLACQH
    61  AFCAICLKLL LCVQDNTWSI TCPLCRKVTA VPGGLICSLR DHEAVVGQLA QPCTEVSLCP
   121  QGLVDPADLA AGHPSLVGED GQDEVSANHV AARRLAAHLL LLALLIILIG PFIYPGVLRW
   181  VLTFIIALAL LMSTLFCCLP STRGSCWPSS RTLFCREQKH SHISSIA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF186 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 123 nTPM

Expression across tissuesHPA

Tissue

duodenum: 123 nTPM
epididymis: 64 nTPM
small intestine: 42 nTPM
pancreas: 42 nTPM
kidney: 25 nTPM
colon: 18 nTPM

Single-cell type

epididymal principal cells: 331 nCPM
enteric stem cells: 129 nCPM
enteric transient amplifying cells: 91 nCPM
colonocytes: 81 nCPM
neuroendocrine cells: 64 nCPM
paneth cells: 46 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 0.2 nTPM
pons: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM
cerebral cortex: 0 nTPM
choroid plexus: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.39
gnomAD pLI: 0.08
gnomAD missense Z: -0.16
DepMap mean gene effect: 0.08
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF186 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

BNIP1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF186 as an antibody target. Whether an autoantibody or antibody against RNF186 could matter depends on whether native RNF186 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF186 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF186 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...