Seroatlas · Human Serome Atlas

RNF168

E3 ubiquitin-protein ligase RNF168

Also known as: FLJ35794, RN168_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8IYW5
Gene
RNF168
Ensembl
ENSG00000163961
Chromosome
3
Canonical length
571 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene encodes an E3 ubiquitin ligase protein that contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The protein is involved in DNA double-strand break (DSB) repair. Mutations in this gene result in Riddle syndrome. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

571 residues, UniProt reviewed canonical sequence.

>Q8IYW5|RNF168
     1  MALPKDAIPS LSECQCGICM EILVEPVTLP CNHTLCKPCF QSTVEKASLC CPFCRRRVSS
    61  WTRYHTRRNS LVNVELWTII QKHYPRECKL RASGQESEEV ADDYQPVRLL SKPGELRREY
   121  EEEISKVAAE RRASEEEENK ASEEYIQRLL AEEEEEEKRQ AEKRRRAMEE QLKSDEELAR
   181  KLSIDINNFC EGSISASPLN SRKSDPVTPK SEKKSKNKQR NTGDIQKYLT PKSQFGSASH
   241  SEAVQEVRKD SVSKDIDSSD RKSPTGQDTE IEDMPTLSPQ ISLGVGEQGA DSSIESPMPW
   301  LCACGAEWYH EGNVKTRPSN HGKELCVLSH ERPKTRVPYS KETAVMPCGR TESGCAPTSG
   361  VTQTNGNNTG ETENEESCLL ISKEISKRKN QESSFEAVKD PCFSAKRRKV SPESSPDQEE
   421  TEINFTQKLI DLEHLLFERH KQEEQDRLLA LQLQKEVDKE QMVPNRQKGS PDEYHLRATS
   481  SPPDKVLNGQ RKNPKDGNFK RQTHTKHPTP ERGSRDKNRQ VSLKMQLKQS VNRRKMPNST
   541  RDHCKVSKSA HSLQPSISQK SVFQMFQRCT K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF168 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.6
Highest tissue expression
19 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 19 nTPM
  • skeletal muscle: 12 nTPM
  • skin: 11 nTPM
  • testis: 10 nTPM
  • liver: 9.7 nTPM
  • vagina: 8.9 nTPM

Single-cell type

  • late spermatids: 735 nCPM
  • early spermatids: 312 nCPM
  • neutrophil progenitors: 196 nCPM
  • basal keratinocytes: 160 nCPM
  • suprabasal keratinocytes: 160 nCPM
  • urothelial cells: 156 nCPM

Immune cell

  • basophil: 30 nTPM
  • gdT-cell: 12 nTPM
  • non-classical monocyte: 12 nTPM
  • MAIT T-cell: 11 nTPM
  • memory CD4 T-cell: 10 nTPM
  • naive CD8 T-cell: 9.3 nTPM

Brain region

  • pons: 20 nTPM
  • medulla oblongata: 20 nTPM
  • hypothalamus: 19 nTPM
  • cerebellum: 19 nTPM
  • white matter: 19 nTPM
  • midbrain: 18 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RNF168.

Disease | AllUniProt

Conditions RNF168 is implicated in, by any mechanism.

Disease | GeneticClinVar

35 pathogenic / likely-pathogenic of 399 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.2
gnomAD pLI
0
gnomAD missense Z
-0.39
DepMap mean gene effect
-0.51
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF168 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF168 as an antibody target. Whether an autoantibody or antibody against RNF168 could matter depends on whether native RNF168 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF168 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF168 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF168. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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