Seroatlas · Human Serome Atlas

RNF128

E3 ubiquitin-protein ligase RNF128

Also known as: FLJ23516, GRAIL, RN128_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8TEB7
Gene
RNF128
Ensembl
ENSG00000133135
Chromosome
X
Canonical length
428 aa
Protein class
Enzymes, Metabolic proteins, Predicted membrane proteins

OverviewNCBI Gene

The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

428 residues, UniProt reviewed canonical sequence.

>Q8TEB7|RNF128
     1  MGPPPGAGVS CRGGCGFSRL LAWCFLLALS PQAPGSRGAE AVWTAYLNVS WRVPHTGVNR
    61  TVWELSEEGV YGQDSPLEPV AGVLVPPDGP GALNACNPHT NFTVPTVWGS TVQVSWLALI
   121  QRGGGCTFAD KIHLAYERGA SGAVIFNFPG TRNEVIPMSH PGAVDIVAIM IGNLKGTKIL
   181  QSIQRGIQVT MVIEVGKKHG PWVNHYSIFF VSVSFFIITA ATVGYFIFYS ARRLRNARAQ
   241  SRKQRQLKAD AKKAIGRLQL RTLKQGDKEI GPDGDSCAVC IELYKPNDLV RILTCNHIFH
   301  KTCVDPWLLE HRTCPMCKCD ILKALGIEVD VEDGSVSLQV PVSNEISNSA SSHEEDNRSE
   361  TASSGYASVQ GTDEPPLEEH VQSTNESLQL VNHEANSVAV DVIPHVDNPT FEEDETPNQE
   421  TAVREIKS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF128 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
117 nTPM

Expression across tissuesHPA

Tissue

  • liver: 117 nTPM
  • duodenum: 58 nTPM
  • small intestine: 57 nTPM
  • stomach: 44 nTPM
  • adrenal gland: 33 nTPM
  • gallbladder: 31 nTPM

Single-cell type

  • foveolar cells: 208 nCPM
  • parietal cells: 117 nCPM
  • gonadotrophs: 113 nCPM
  • enterocytes: 92 nCPM
  • hepatocytes: 89 nCPM
  • urothelial cells: 61 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • hypothalamus: 8.9 nTPM
  • pons: 6.7 nTPM
  • medulla oblongata: 5.3 nTPM
  • basal ganglia: 5.1 nTPM
  • hippocampal formation: 5 nTPM
  • cerebral cortex: 4.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.67
gnomAD pLI
0.23
gnomAD missense Z
0.67
DepMap mean gene effect
0
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF128 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF128 as an antibody target. Whether an autoantibody or antibody against RNF128 could matter depends on whether native RNF128 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF128 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF128 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF128. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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