RLF

Protein identityUniProt · HPA

UniProt accession

Q13129

Gene

RLF

Ensembl

ENSG00000117000

Chromosome

1

Canonical length

1914 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Nucleoli

OverviewNCBI Gene

Predicted to enable DNA binding activity and DNA-binding transcription activator activity, RNA polymerase II-specific. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within negative regulation of heterochromatin formation. Located in extracellular exosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1914 residues, UniProt reviewed canonical sequence.

>Q13129|RLF
     1  MADGKGDAAA VAGAGAEAPA VAGAGDGVET ESMVRGHRPV SPAPGASGLR PCLWQLETEL
    61  REQEVSEVSS LNYCRSFCQT LLQYASNKNA SEHIVYLLEV YRLAIQSFAS ARPYLTTECE
   121  DVLLVLGRLV LSCFELLLSV SESELPCEVW LPFLQSLQES HDALLEFGNN NLQILVHVTK
   181  EGVWKNPVLL KILSQQPVET EEVNKLIAQE GPSFLQMRIK HLLKSNCIPQ ATALSKLCAE
   241  SKEISNVSSF QQAYITCLCS MLPNEDAIKE IAKVDCKEVL DIICNLESEG QDNTAFVLCT
   301  TYLTQQLQTA SVYCSWELTL FWSKLQRRID PSLDTFLERC RQFGVIAKTQ QHLFCLIRVI
   361  QTEAQDAGLG VSILLCVRAL QLRSSEDEEM KASVCKTIAC LLPEDLEVRR ACQLTEFLIE
   421  PSLDGFNMLE ELYLQPDQKF DEENAPVPNS LRCELLLALK AHWPFDPEFW DWKTLKRHCH
   481  QLLGQEASDS DDDLSGYEMS INDTDVLESF LSDYDEGKED KQYRRRDLTD QHKEKRDKKP
   541  IGSSERYQRW LQYKFFCLLC KRECIEARIL HHSKMHMEDG IYTCPVCIKK FKRKEMFVPH
   601  VMEHVKMPPS RRDRSKKKLL LKGSQKGICP KSPSAIPEQN HSLNDQAKGE SHEYVTFSKL
   661  EDCHLQDRDL YPCPGTDCSR VFKQFKYLSV HLKAEHQNND ENAKHYLDMK NRREKCTYCR
   721  RHFMSAFHLR EHEQVHCGPQ PYMCVSIDCY ARFGSVNELL NHKQKHDDLR YKCELNGCNI
   781  VFSDLGQLYH HEAQHFRDAS YTCNFLGCKK FYYSKIEYQN HLSMHNVENS NGDIKKSVKL
   841  EESATGEKQD CINQPHLLNQ TDKSHLPEDL FCAESANSQI DTETAENLKE NSDSNSSDQL
   901  SHSSSASMNE ELIDTLDHSE TMQDVLLSNE KVFGPSSLKE KCSSMAVCFD GTKFTCGFDG
   961  CGSTYKNARG MQKHLRKVHP YHFKPKKIKT KDLFPSLGNE HNQTTEKLDA EPKPCSDTNS
  1021  DSPDEGLDHN IHIKCKREHQ GYSSESSICA SKRPCTEDTM LELLLRLKHL SLKNSITHGS
  1081  FSGSLQGYPS SGAKSLQSVS SISDLNFQNQ DENMPSQYLA QLAAKPFFCE LQGCKYEFVT
  1141  REALLMHYLK KHNYSKEKVL QLTMFQHRYS PFQCHICQRS FTRKTHLRIH YKNKHQIGSD
  1201  RATHKLLDNE KCDHEGPCSV DRLKGDCSAE LGGDPSSNSE KPHCHPKKDE CSSETDLESS
  1261  CEETESKTSD ISSPIGSHRE EQEGREGRGS RRTVAKGNLC YILNKYHKPF HCIHKTCNSS
  1321  FTNLKGLIRH YRTVHQYNKE QLCLEKDKAR TKRELVKCKK IFACKYKECN KRFLCSKALA
  1381  KHCSDSHNLD HIEEPKVLSE AGSAARFSCN QPQCPAVFYT FNKLKHHLME QHNIEGEIHS
  1441  DYEIHCDLNG CGQIFTHRSN YSQHVYYRHK DYYDDLFRSQ KVANERLLRS EKVCQTADTQ
  1501  GHEHQTTRRS FNAKSKKCGL IKEKKAPISF KTRAEALHMC VEHSEHTQYP CMVQGCLSVV
  1561  KLESSIVRHY KRTHQMSSAY LEQQMENLVV CVKYGTKIKE EPPSEADPCI KKEENRSCES
  1621  ERTEHSHSPG DSSAPIQNTD CCHSSERDGG QKGCIESSSV FDADTLLYRG TLKCNHSSKT
  1681  TSLEQCNIVQ PPPPCKIENS IPNPNGTESG TYFTSFQLPL PRIKESETRQ HSSGQENTVK
  1741  NPTHVPKENF RKHSQPRSFD LKTYKPMGFE SSFLKFIQES EEKEDDFDDW EPSEHLTLSN
  1801  SSQSSNDLTG NVVANNMVND SEPEVDIPHS SSDSTIHENL TAIPPLIVAE TTTVPSLENL
  1861  RVVLDKALTD CGELALKQLH YLRPVVVLER SKFSTPILDL FPTKKTDELC VGSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RLF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

31 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.04

gnomAD pLI

1

gnomAD missense Z

3.46

DepMap mean gene effect

-0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of heterochromatin formation
• positive regulation of DNA-templated transcription
• positive regulation of transcription by RNA polymerase II
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA binding
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• zinc ion binding

Cellular components

• nucleus

Protein domainsUniProt · Pfam · InterPro

• Zinc finger C2H2-type
• Zinc finger C2H2 superfamily
• Glycosyl Hydrolase and Zinc Finger Regulators
• Zinc finger protein Rlf/292/654, TPR repeats
• Zinc finger protein 292/Rlf, C2H2-type zinc finger
• Zinc finger, C2H2 type
• ZN292-like C2H2 zinc finger
• Zinc finger protein Rlf-like, TPR repeats
• Zinc finger protein 292, C2H2-type zinc finger

KeywordsUniProt

• DNA-binding
• Isopeptide bond
• Metal-binding
• Nucleus
• Phosphoprotein
• Repeat
• Transcription
• Transcription regulation
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of RLF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RIT1
• RIT2