RFK

Protein identityUniProt · HPA

UniProt accession

Q969G6

Gene

RFK

Ensembl

ENSG00000135002

Chromosome

9

Canonical length

155 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Golgi apparatus

OverviewNCBI Gene

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]

Canonical amino-acid sequenceUniProt

155 residues, UniProt reviewed canonical sequence.

>Q969G6|RFK
     1  MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW ASVGSGDVHK
    61  MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV GYLRPEKNFD SLESLISAIQ
   121  GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK IMNGH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

32 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1

gnomAD pLI

0.06

gnomAD missense Z

0.49

DepMap mean gene effect

-0.31

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• flavin adenine dinucleotide biosynthetic process
• reactive oxygen species metabolic process
• riboflavin metabolic process
• FMN biosynthetic process
• positive regulation of NAD(P)H oxidase activity
• riboflavin biosynthetic process

Molecular functions

• ATP binding
• metal ion binding
• riboflavin kinase activity

Cellular components

• cytoplasm
• cytosol
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Riboflavin kinase domain, bacterial/eukaryotic
• Riboflavin kinase domain superfamily
• Riboflavin kinase
• Riboflavin kinase

KeywordsUniProt

• ATP-binding
• Cytoplasm
• Flavoprotein
• FMN
• Kinase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Transferase
• Zinc

InteractionsUniProt · HPA

Protein binding partners of RFK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TRADD
• TNFRSF1A