Seroatlas · Human Serome Atlas

RBAK

RB-associated KRAB zinc finger protein

Also known as: RBAK_HUMAN, ZNF769

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NYW8
Gene: RBAK
Ensembl: ENSG00000146587
Chromosome: 7
Canonical length: 714 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a nuclear protein which interacts with the tumor suppressor retinoblastoma 1. The two interacting proteins are thought to act as a transcriptional repressor for promoters which are activated by the E2F1 transcription factor. This protein contains a Kruppel-associated box (KRAB), which is a transcriptional repressor motif. Read-through transcripts that include exons from the downstream gene LOC389458 are expressed from this locus. [provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

714 residues, UniProt reviewed canonical sequence.

>Q9NYW8|RBAK
     1  MNTLQGPVSF KDVAVDFTQE EWQQLDPDEK ITYRDVMLEN YSHLVSVGYD TTKPNVIIKL
    61  EQGEEPWIMG GEFPCQHSPE AWRVDDLIER IQENEDKHSR QAACINSKTL TEEKENTFSQ
   121  IYMETSLVPS SIIAHNCVSC GKNLESISQL ISSDGSYART KPDECNECGK TYHGEKMCEF
   181  NQNGDTYSHN EENILQKISI LEKPFEYNEC MEALDNEAVF IAHKRAYIGE KPYEWNDSGP
   241  DFIQMSNFNA YQRSQMEMKP FECSECGKSF CKKSKFIIHQ RAHTGEKPYE CNVCGKSFSQ
   301  KGTLTVHRRS HLEEKPYKCN ECGKTFCQKL HLTQHLRTHS GEKPYECSEC GKTFCQKTHL
   361  TLHQRNHSGE RPYPCNECGK SFSRKSALSD HQRTHTGEKL YKCNECGKSY YRKSTLITHQ
   421  RTHTGEKPYQ CSECGKFFSR VSYLTIHYRS HLEEKPYECN ECGKTFNLNS AFIRHRKVHT
   481  EEKSHECSEC GKFSQLYLTD HHTAHLEEKP YECNECGKTF LVNSAFDGHQ PLPKGEKSYE
   541  CNVCGKLFNE LSYYTEHYRS HSEEKPYGCS ECGKTFSHNS SLFRHQRVHT GEKPYECYEC
   601  GKFFSQKSYL TIHHRIHSGE KPYECSKCGK VFSRMSNLTV HYRSHSGEKP YECNECGKVF
   661  SQKSYLTVHY RTHSGEKPYE CNECGKKFHH RSAFNSHQRI HRRGNMNVLD VENL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RBAK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 7.8 nTPM

Expression across tissuesHPA

Tissue

retina: 7.8 nTPM
cerebellum: 6.8 nTPM
parathyroid gland: 6.5 nTPM
tonsil: 6.3 nTPM
liver: 6.2 nTPM
lymph node: 6 nTPM

Single-cell type

choroid plexus epithelial cells: 18 nCPM
other brain neurons: 15 nCPM
oligodendrocytes: 15 nCPM
oligodendrocyte progenitor cells: 15 nCPM
astrocytes: 15 nCPM
brain inhibitory neurons: 15 nCPM

Immune cell

MAIT T-cell: 0.7 nTPM
gdT-cell: 0.5 nTPM
naive CD4 T-cell: 0.5 nTPM
NK-cell: 0.5 nTPM
eosinophil: 0.4 nTPM
intermediate monocyte: 0.4 nTPM

Brain region

cerebellum: 15 nTPM
cerebral cortex: 12 nTPM
midbrain: 12 nTPM
hypothalamus: 11 nTPM
medulla oblongata: 11 nTPM
white matter: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.92
gnomAD pLI: 0
gnomAD missense Z: 0.09
DepMap mean gene effect: 0.21
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RBAK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RBAK as an antibody target. Whether an autoantibody or antibody against RBAK could matter depends on whether native RBAK is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RBAK is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RBAK as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...