RASGRP3

Protein identityUniProt · HPA

UniProt accession

Q8IV61

Gene

RASGRP3

Ensembl

ENSG00000152689

Chromosome

2

Canonical length

690 aa

Protein class

Predicted intracellular proteins, RAS pathway related proteins

Subcellular location

Golgi apparatus,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

690 residues, UniProt reviewed canonical sequence.

>Q8IV61|RASGRP3
     1  MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE LAEKLLCMYR
    61  NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE EFREVASQLG YEKHVSLIDI
   121  SSIPSYDWMR RVTQRKKVSK KGKACLLFDH LEPIELAEHL TFLEHKSFRR ISFTDYQSYV
   181  IHGCLENNPT LERSIALFNG ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT
   241  LMAVVGGLSH SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP
   301  ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL EPNMDLINLL
   361  TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH
   421  IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR
   481  AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL
   541  VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK
   601  ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV
   661  HAGVDVVDRG TEFELDQDEG EETRQDGEDG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RASGRP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

47 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0.01

gnomAD missense Z

0.5

DepMap mean gene effect

0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• MAPK cascade
• Ras protein signal transduction
• small GTPase-mediated signal transduction

Molecular functions

• calcium ion binding
• diacylglycerol binding
• GTPase activator activity
• guanyl-nucleotide exchange factor activity
• kinase binding
• small GTPase binding
• zinc ion binding

Cellular components

• guanyl-nucleotide exchange factor complex
• perinuclear region of cytoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Ras-like guanine nucleotide exchange factor, N-terminal
• Ras guanine-nucleotide exchange factors catalytic domain
• EF-hand domain
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• Ras-like guanine nucleotide exchange factor
• EF-hand domain pair
• EF-Hand 1, calcium-binding site
• Diacylglycerol/phorbol-ester binding
• Ras guanine nucleotide exchange factor domain superfamily
• Ras guanine-nucleotide exchange factor, catalytic domain superfamily
• C1-like domain superfamily
• Phorbol esters/diacylglycerol binding domain (C1 domain)
• RasGEF domain
• EF-hand domain pair

KeywordsUniProt

• Calcium
• Guanine-nucleotide releasing factor
• Metal-binding
• Repeat
• Zinc
• Zinc-finger