Seroatlas · Human Serome Atlas

RASA3

Ras GTPase-activating protein 3

Also known as: GAP1IP4BP, GAPIII, RASA3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14644
Gene: RASA3
Ensembl: ENSG00000185989
Chromosome: 13
Canonical length: 834 aa
Protein class: Plasma proteins, Predicted intracellular proteins, RAS pathway related proteins

OverviewNCBI Gene

This gene encodes a protein that binds inositol 1,3,4,5-tetrakisphosphate and stimulates the GTPase activity of Ras p21. This protein functions as a negative regulator of the Ras signalling pathway. It is localized to the cell membrane via a pleckstrin homology (PH) domain in the C-terminal region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Canonical amino-acid sequenceUniProt

834 residues, UniProt reviewed canonical sequence.

>Q14644|RASA3
     1  MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT KIVEKSLCPF
    61  YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE DLQKYHNRDT WFQLQHVDAD
   121  SEVQGKVHLE LRLSEVITDT GVVCHKLATR IVECQGLPIV NGQCDPYATV TLAGPFRSEA
   181  KKTKVKRKTN NPQFDEVFYF EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD
   241  EFLGELRIPL KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY
   301  SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP FISAIASAEV
   361  KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI EEICQSHKPC EIDPVKLKDG
   421  ENLENNMENL RQYVDRVFHA ITESGVSCPT VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS
   481  FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT
   541  FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK
   601  RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ PERALYIQAN
   661  NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR APSDSAPGCS PCTGGLPANI
   721  QLDIDGDRET ERIYSLFNLY MSKLEKMQEA CGSKSVYDGP EQEEYSTFVI DDPQETYKTL
   781  KQVIAGVGAL EQEHAQYKRD KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RASA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

adipose tissue: 30 nTPM
breast: 22 nTPM
spleen: 21 nTPM
cervix: 20 nTPM
blood vessel: 20 nTPM
lung: 19 nTPM

Single-cell type

microglia: 153 nCPM
ependymal cells: 85 nCPM
nk-cells: 69 nCPM
bergmann glia: 49 nCPM
astrocytes: 38 nCPM
other brain neurons: 32 nCPM

Immune cell

T-reg: 19 nTPM
memory CD8 T-cell: 17 nTPM
gdT-cell: 17 nTPM
total PBMC: 17 nTPM
basophil: 17 nTPM
naive CD8 T-cell: 17 nTPM

Brain region

white matter: 28 nTPM
cerebral cortex: 22 nTPM
thalamus: 19 nTPM
medulla oblongata: 19 nTPM
pons: 17 nTPM
midbrain: 17 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RASA3.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 169 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Short stature

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.71
gnomAD pLI: 0
gnomAD missense Z: 1.68
DepMap mean gene effect: 0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RASA3 as an antibody target. Whether an autoantibody or antibody against RASA3 could matter depends on whether native RASA3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RASA3 is annotated at the cell surface, where native RASA3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RASA3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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