RAMP1

Protein identityUniProt · HPA

UniProt accession

O60894

Gene

RAMP1

Ensembl

ENSG00000132329

Chromosome

2

Canonical length

148 aa

Protein class

FDA approved drug targets, Predicted membrane proteins, Transporters

Subcellular location

Nucleoplasm,Nuclear membrane

OverviewNCBI Gene

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Canonical amino-acid sequenceUniProt

148 residues, UniProt reviewed canonical sequence.

>O60894|RAMP1
     1  MARALCRLPR RGLWLLLAHH LFMTTACQEA NYGALLRELC LTQFQVDMEA VGETLWCDWG
    61  RTIRSYRELA DCTWHMAEKL GCFWPNAEVD RFFLAVHGRY FRSCPISGRA VRDPPGSILY
   121  PFIVVPITVT LLVTALVVWQ SKRTEGIV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAMP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.43

Highest tissue expression

694 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.12

gnomAD pLI

0.13

gnomAD missense Z

-0.55

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adenylate cyclase-activating G protein-coupled receptor signaling pathway
• amylin receptor 1 signaling pathway
• amylin receptor signaling pathway
• angiogenesis
• calcitonin gene-related peptide receptor signaling pathway
• calcium ion transport
• cellular response to hormone stimulus
• G protein-coupled receptor signaling pathway
• intracellular protein transport
• positive regulation of glycoprotein biosynthetic process
• protein localization to plasma membrane
• protein transport
• receptor internalization
• regulation of G protein-coupled receptor signaling pathway

Molecular functions

• amylin receptor activity
• calcitonin gene-related peptide receptor activity
• coreceptor activity
• calcitonin gene-related peptide binding

Cellular components

• cell surface
• CGRP receptor complex
• plasma membrane
• receptor complex

Protein domainsUniProt · Pfam · InterPro

• Receptor activity modifying protein
• RAMP domain superfamily
• Receptor activity modifying family

KeywordsUniProt

• Cell membrane
• Disulfide bond
• Membrane
• Receptor
• Signal
• Transmembrane
• Transmembrane helix
• Transport

InteractionsUniProt · HPA

Protein binding partners of RAMP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CALCRL