PRPS1L1

Protein identityUniProt · HPA

UniProt accession

P21108

Gene

PRPS1L1

Ensembl

ENSG00000229937

Chromosome

7

Canonical length

318 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Vesicles

Quaternary structure

Homodimer

OverviewNCBI Gene

This intronless gene is specifically expressed in the testis, and encodes a protein that is highly homologous to the two subunits of phosphoribosylpyrophosphate synthetase encoded by human X-linked genes, PRPS1 and PRPS2. These enzymes convert pyrimidine, purine or pyridine bases to the corresponding ribonucleoside monophosphates. In vitro transcription/translation and site-directed mutagenesis studies indicate that translation of this mRNA initiates exclusively at a non-AUG (ACG) codon. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

318 residues, UniProt reviewed canonical sequence.

>P21108|PRPS1L1
     1  MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIDESVRG EDVYIVQSGC
    61  GEINDSLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRSPISAK LVANMLSIAG
   121  ADHIITMDLH ASQIQGFFDI PVDNLYAEPT VLKWIRENIP EWKNCIIVSP DAGGAKRVTS
   181  IADQLNVDFA LIHKERKKAN EVDCIVLVGD VNDRVAILVD DMADTCVTIC LAADKLLSAG
   241  ATRVYAILTH GIFSGPAISR INTACFEAVV VTNTIPQDEK MKHCSKIRVI DISMILAEAI
   301  RRTHNGESVS YLFSHVPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRPS1L1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

29 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.79

gnomAD pLI

0.29

gnomAD missense Z

-0.44

DepMap mean gene effect

0.14

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 5-phosphoribose 1-diphosphate biosynthetic process
• male gonad development
• purine nucleotide biosynthetic process
• ribonucleoside monophosphate biosynthetic process

Molecular functions

• ATP binding
• kinase activity
• magnesium ion binding
• protein homodimerization activity
• ribose phosphate diphosphokinase activity

Cellular components

• cytoplasm
• ribose phosphate diphosphokinase complex

Protein domainsUniProt · Pfam · InterPro

• Phosphoribosyltransferase domain
• Phosphoribosyl pyrophosphate synthetase, conserved site
• Ribose-phosphate pyrophosphokinase
• Phosphoribosyltransferase-like
• Ribose-phosphate pyrophosphokinase, N-terminal domain
• Ribose-phosphate pyrophosphokinase, bacterial-type
• N-terminal domain of ribose phosphate pyrophosphokinase
• Phosphoribosyl synthetase-associated domain

KeywordsUniProt

• ATP-binding
• Kinase
• Magnesium
• Metal-binding
• Nucleotide biosynthesis
• Nucleotide-binding
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PRPS1L1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PRPS1