Seroatlas · Human Serome Atlas

PRKD3

Serine/threonine-protein kinase D3

Also known as: EPK2, KPCD3_HUMAN, PRKCN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O94806
Gene
PRKD3
Ensembl
ENSG00000115825
Chromosome
2
Canonical length
890 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Plasma membrane,Basal body,Cytosol

OverviewNCBI Gene

This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015]

Canonical amino-acid sequenceUniProt

890 residues, UniProt reviewed canonical sequence.

>O94806|PRKD3
     1  MSANNSPPSA QKSVLPTAIP AVLPAASPCS SPKTGLSARL SNGSFSAPSL TNSRGSVHTV
    61  SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC GFFGMYDKIL LFRHDMNSEN
   121  ILQLITSADE IHEGDLVEVV LSALATVEDF QIRPHTLYVH SYKAPTFCDY CGEMLWGLVR
   181  QGLKCEGCGL NYHKRCAFKI PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP EYVALPSEES
   241  HVHQEPSKRI PSWSGRPIWM EKMVMCRVKV PHTFAVHSYT RPTICQYCKR LLKGLFRQGM
   301  QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PSSLGTDTDI PMDIDNNDIN SDSSRGLDDT
   361  EEPSPPEDKM FFLDPSDLDV ERDEEAVKTI SPSTSNNIPL MRVVQSIKHT KRKSSTMVKE
   421  GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN ESGSKYYKEI PLSEILRISS PRDFTNISQG
   481  SNPHCFEIIT DTMVYFVGEN NGDSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV
   541  CTSPGQGKDH KDLSTSISVS NCQIQENVDI STVYQIFADE VLGSGQFGIV YGGKHRKTGR
   601  DVAIKVIDKM RFPTKQESQL RNEVAILQNL HHPGIVNLEC MFETPERVFV VMEKLHGDML
   661  EMILSSEKSR LPERITKFMV TQILVALRNL HFKNIVHCDL KPENVLLASA EPFPQVKLCD
   721  FGFARIIGEK SFRRSVVGTP AYLAPEVLRS KGYNRSLDMW SVGVIIYVSL SGTFPFNEDE
   781  DINDQIQNAA FMYPPNPWRE ISGEAIDLIN NLLQVKMRKR YSVDKSLSHP WLQDYQTWLD
   841  LREFETRIGE RYITHESDDA RWEIHAYTHN LVYPKHFIMA PNPDDMEEDP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRKD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
39 nTPM

Expression across tissuesHPA

Tissue

  • retina: 39 nTPM
  • lymph node: 27 nTPM
  • tonsil: 27 nTPM
  • spleen: 22 nTPM
  • testis: 22 nTPM
  • thymus: 19 nTPM

Single-cell type

  • melanocytes: 342 nCPM
  • rod photoreceptor cells: 285 nCPM
  • somatotrophs: 250 nCPM
  • myonuclei: 209 nCPM
  • late spermatids: 187 nCPM
  • adrenal cortex cells: 183 nCPM

Immune cell

  • naive B-cell: 15 nTPM
  • naive CD8 T-cell: 11 nTPM
  • memory B-cell: 11 nTPM
  • naive CD4 T-cell: 9.7 nTPM
  • gdT-cell: 9.6 nTPM
  • memory CD4 T-cell: 8.6 nTPM

Brain region

  • thalamus: 18 nTPM
  • cerebellum: 16 nTPM
  • medulla oblongata: 16 nTPM
  • hypothalamus: 16 nTPM
  • spinal cord: 16 nTPM
  • midbrain: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.46
gnomAD pLI
0.01
gnomAD missense Z
1.51
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRKD3 as an antibody target. Whether an autoantibody or antibody against PRKD3 could matter depends on whether native PRKD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRKD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRKD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRKD3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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