PRIM2

Protein identityUniProt · HPA

UniProt accession

P49643

Gene

PRIM2

Ensembl

ENSG00000146143

Chromosome

6

Canonical length

509 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]

Canonical amino-acid sequenceUniProt

509 residues, UniProt reviewed canonical sequence.

>P49643|PRIM2
     1  MEFSGRKWRK LRLAGDQRNA SYPHCLQFYL QPPSENISLI EFENLAIDRV KLLKSVENLG
    61  VSYVKGTEQY QSKLESELRK LKFSYRENLE DEYEPRRRDH ISHFILRLAY CQSEELRRWF
   121  IQQEMDLLRF RFSILPKDKI QDFLKDSQLQ FEAISDEEKT LREQEIVASS PSLSGLKLGF
   181  ESIYKIPFAD ALDLFRGRKV YLEDGFAYVP LKDIVAIILN EFRAKLSKAL ALTARSLPAV
   241  QSDERLQPLL NHLSHSYTGQ DYSTQGNVGK ISLDQIDLLS TKSFPPCMRQ LHKALRENHH
   301  LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIKGKMDPDK FDKGYSYNIR HSFGKEGKRT
   361  DYTPFSCLKI ILSNPPSQGD YHGCPFRHSD PELLKQKLQS YKISPGGISQ ILDLVKGTHY
   421  QVACQKYFEM IHNVDDCGFS LNHPNQFFCE SQRILNGGKD IKKEPIQPET PQPKPSVQKT
   481  KDASSALASL NSSLEMDMEG LEDYFSEDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRIM2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

30 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.03

gnomAD pLI

0

gnomAD missense Z

0.76

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA replication initiation
• DNA replication, synthesis of primer

Molecular functions

• 4 iron, 4 sulfur cluster binding
• DNA binding
• DNA/RNA hybrid binding
• metal ion binding

Cellular components

• alpha DNA polymerase:primase complex
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• DNA primase large subunit, eukaryotic/archaeal
• DNA primase, large subunit, eukaryotic
• DNA primase, large subunit C-terminal domain, eukaryotic and archaeal
• Eukaryotic and archaeal DNA primase, large subunit C-terminal domain
• Eukaryotic and archaeal DNA primase, large subunit N-terminal domain

KeywordsUniProt

• 4Fe-4S
• DNA replication
• DNA-binding
• Iron
• Iron-sulfur
• Metal-binding
• Phosphoprotein
• Primosome

InteractionsUniProt · HPA

Protein binding partners of PRIM2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PRIM1