Seroatlas · Human Serome Atlas

PRIM1

DNA primase small subunit

Also known as: PRI1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P49642
Gene: PRIM1
Ensembl: ENSG00000198056
Chromosome: 12
Canonical length: 420 aa
Protein class: Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins

OverviewNCBI Gene

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

420 residues, UniProt reviewed canonical sequence.

>P49642|PRIM1
     1  METFDPTELP ELLKLYYRRL FPYSQYYRWL NYGGVIKNYF QHREFSFTLK DDIYIRYQSF
    61  NNQSDLEKEM QKMNPYKIDI GAVYSHRPNQ HNTVKLGAFQ AQEKELVFDI DMTDYDDVRR
   121  CCSSADICPK CWTLMTMAIR IIDRALKEDF GFKHRLWVYS GRRGVHCWVC DESVRKLSSA
   181  VRSGIVEYLS LVKGGQDVKK KVHLSEKIHP FIRKSINIIK KYFEEYALVN QDILENKESW
   241  DKILALVPET IHDELQQSFQ KSHNSLQRWE HLKKVASRYQ NNIKNDKYGP WLEWEIMLQY
   301  CFPRLDINVS KGINHLLKSP FSVHPKTGRI SVPIDLQKVD QFDPFTVPTI SFICRELDAI
   361  STNEEEKEEN EAESDVKHRT RDYKKTSLAP YVKVFEHFLE NLDKSRKGEL LKKSDLQKDF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRIM1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 26 nTPM
thymus: 15 nTPM
testis: 11 nTPM
tonsil: 11 nTPM
lymph node: 10 nTPM
rectum: 9.6 nTPM

Single-cell type

early primary spermatocytes: 165 nCPM
differentiating spermatogonia: 84 nCPM
erythrocyte progenitors: 67 nCPM
megakaryocyte progenitors: 43 nCPM
oocytes: 41 nCPM
megakaryocyte-erythroid progenitors: 31 nCPM

Immune cell

naive CD4 T-cell: 11 nTPM
naive B-cell: 8.9 nTPM
memory B-cell: 8.3 nTPM
naive CD8 T-cell: 8.2 nTPM
memory CD4 T-cell: 8 nTPM
T-reg: 7.5 nTPM

Brain region

cerebellum: 9.9 nTPM
white matter: 7.5 nTPM
basal ganglia: 5.7 nTPM
cerebral cortex: 5.4 nTPM
pons: 5.3 nTPM
medulla oblongata: 5.1 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PRIM1.

Disease | AllUniProt

Conditions PRIM1 is implicated in, by any mechanism.

Primordial dwarfism-immunodeficiency-lipodystrophy syndrome (PDIL) MIM:620005

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 64 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Primordial dwarfism-immunodeficiency-lipodystrophy syndrome
Seckel syndrome

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.69
gnomAD pLI: 0
gnomAD missense Z: 2.05
DepMap mean gene effect: -1.77
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

DNA primase, small subunit
DNA primase small subunit
DNA primase, small subunit, eukaryotic/archaeal

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRIM1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

PRIM2

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRIM1 as an antibody target. Whether an autoantibody or antibody against PRIM1 could matter depends on whether native PRIM1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRIM1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRIM1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...