Seroatlas · Human Serome Atlas

PRAG1

Inactive tyrosine-protein kinase PRAG1

Also known as: DKFZp761P0423, NACK, PEAK2, PRAG1_HUMAN, PRAGMIN, SgK223

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q86YV5
Gene
PRAG1
Ensembl
ENSG00000275342
Chromosome
8
Canonical length
1406 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoli,Focal adhesion sites
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014]

Canonical amino-acid sequenceUniProt

1406 residues, UniProt reviewed canonical sequence.

>Q86YV5|PRAG1
     1  MHQTLCLNPE SLKMSACSDF VEHIWKPGSC KNCFCLRSDH QLVAGPPQPR AGSLPPPPRL
    61  PPRPENCRLE DEGVNSSPYS KPTIAVKPTM MSSEASDVWT EANLSAEVSQ VIWRRAPGKL
   121  PLPKQEDAPV VYLGSFRGVQ KPAGPSTSPD GNSRCPPAYT MVGLHNLEPR GERNIAFHPV
   181  SFPEEKAVHK EKPSFPYQDR PSTQESFRQK LAAFAGTTSG CHQGPGPLRE SLPSEDDSDQ
   241  RCSPSGDSEG GEYCSILDCC PGSPVAKAAS QTAGSRGRHG GRDCSPTCWE QGKCSGPAEQ
   301  EKRGPSFPKE CCSQGPTAHP SCLGPKKLSL TSEAAISSDG LSCGSGSGSG SGASSPFVPH
   361  LESDYCSLMK EPAPEKQQDP GCPGVTPSRC LGLTGEPQPP AHPREATQPE PIYAESTKRK
   421  KAAPVPSKSQ AKIEHAAAAQ GQGQVCTGNA WAQKAASGWG RDSPDPTPQV SATITVMAAH
   481  PEEDHRTIYL SSPDSAVGVQ WPRGPVSQNS EVGEEETSAG QGLSSRESHA HSASESKPKE
   541  RPAIPPKLSK SSPVGSPVSP SAGGPPVSPL ADLSDGSSGG SSIGPQPPSQ GPADPAPSCR
   601  TNGVAISDPS RCPQPAASSA SEQRRPRFQA GTWSRQCRIE EEEEVEQELL SHSWGRETKN
   661  GPTDHSNSTT WHRLHPTDGS SGQNSKVGTG MSKSASFAFE FPKDRSGIET FSPPPPPPKS
   721  RHLLKMNKSS SDLEKVSQGS AESLSPSFRG VHVSFTTGST DSLASDSRTC SDGGPSSELA
   781  HSPTNSGKKL FAPVPFPSGS TEDVSPSGPQ QPPPLPQKKI VSRAASSPDG FFWTQGSPKP
   841  GTASPKLNLS HSETNVHDES HFSYSLSPGN RHHPVFSSSD PLEKAFKGSG HWLPAAGLAG
   901  NRGGCGSPGL QCKGAPSASS SQLSVSSQAS TGSTQLQLHG LLSNISSKEG TYAKLGGLYT
   961  QSLARLVAKC EDLFMGGQKK ELHFNENNWS LFKLTCNKPC CDSGDAIYYC ATCSEDPGST
  1021  YAVKICKAPE PKTVSYCSPS VPVHFNIQQD CGHFVASVPS SMLSSPDAPK DPVPALPTHP
  1081  PAQEQDCVVV ITREVPHQTA SDFVRDSAAS HQAEPEAYER RVCFLLLQLC NGLEHLKEHG
  1141  IIHRDLCLEN LLLVHCTLQA GPGPAPAPAP APAPAAAAPP CSSAAPPAGG TLSPAAGPAS
  1201  PEGPREKQLP RLIISNFLKA KQKPGGTPNL QQKKSQARLA PEIVSASQYR KFDEFQTGIL
  1261  IYELLHQPNP FEVRAQLRER DYRQEDLPPL PALSLYSPGL QQLAHLLLEA DPIKRIRIGE
  1321  AKRVLQCLLW GPRRELVQQP GTSEEALCGT LHNWIDMKRA LMMMKFAEKA VDRRRGVELE
  1381  DWLCCQYLAS AEPGALLQSL KLLQLL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRAG1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.59
Highest tissue expression
41 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 41 nTPM
  • thyroid gland: 22 nTPM
  • small intestine: 11 nTPM
  • duodenum: 11 nTPM
  • cerebral cortex: 9 nTPM
  • breast: 8.9 nTPM

Single-cell type

  • brain excitatory neurons: 105 nCPM
  • microglia: 73 nCPM
  • brain inhibitory neurons: 57 nCPM
  • ependymal cells: 53 nCPM
  • proximal tubule cells: 48 nCPM
  • bergmann glia: 42 nCPM

Immune cell

  • plasmacytoid DC: 1.9 nTPM
  • naive CD4 T-cell: 1.6 nTPM
  • naive CD8 T-cell: 1.3 nTPM
  • memory B-cell: 1.2 nTPM
  • naive B-cell: 1 nTPM
  • eosinophil: 0.9 nTPM

Brain region

  • cerebellum: 81 nTPM
  • basal ganglia: 34 nTPM
  • amygdala: 28 nTPM
  • cerebral cortex: 21 nTPM
  • hippocampal formation: 20 nTPM
  • thalamus: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.48
gnomAD pLI
0
DepMap mean gene effect
0.12
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRAG1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRAG1 as an antibody target. Whether an autoantibody or antibody against PRAG1 could matter depends on whether native PRAG1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRAG1 is annotated at the cell surface, where native PRAG1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label PRAG1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRAG1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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